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In vitro Antibacterial Efficacy of Various Root Canal Sealers Against Enterococcus Faecalis
Maneesha Das1, Prachi Joshi2, Arpita Hasmukhlal Jariwala3
1Professor and Head-in-Charge, Department of Conservative Dentistry and Endodontics, Institute of Dental Sciences, Siksha 'O' Anusandhan Deemed to be University, Bhubaneswar, Odisha, India.
Objective:
The objective of this in vitro study is to evaluate and compare the antibacterial effectiveness of different root canal sealers against Enterococcus faecalis.
Materials And Methods:
The antibacterial efficacy of four root canal sealers-three bioceramic materials (TotalFill Bioceramic Sealer, BioRoot Root Canal Sealer, and MTA Fillapex) and one epoxy resin-based sealer (AH Plus)-against E. faecalis (ATCC 29212) was assessed using a modified direct contact test. Every sealer was made under the manufacturer's instructions, and its antibacterial activity was evaluated against newly mixed and set sealers (1 and 3 days) at various intervals (2, 5, 20, and 60 min). Mitis salivarius agar was used to plate the bacterial suspension, and one-way ANOVA was used for statistical analysis.
Results:
TotalFill Bioceramic Sealer showed the highest antibacterial efficacy, followed by BioRoot Root Canal Sealer, MTA Fillapex, and AH Plus.
Conclusion:
Bioceramic root canal sealers, particularly TotalFill, demonstrate superior antibacterial efficacy against E. faecalis compared to epoxy resin-based sealers such as AH Plus.
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