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Extraction and Analysis of Antimicrobial Compounds from Onosma Bracteatum Using Response Surface Methodology
1Department of Basic Science, College of Medicine, Majmaah University, Majmaah, Saudi Arabia.
Background:
Infectious diseases are the world's leading cause of premature mortality, almost killing 65,000 people every day.
Objective:
The main objective of the study is to find the extraction and analysis of antimicrobial compounds from Onosma Bracteatum using response surface methodology.
Materials And Methods:
ATCC culture samples and antibiotic discs were purchased from PCSIR and scientific markets. All chemicals with reagents of analytical and commercial grades were purchased from Sigma Millipore Chemical Germany, Daejung Chemicals, China, and Unichem England. ONOSMA BRACTEATUM was purchased from the herbal market and its extracts were prepared in the lab.
Results:
The plant extracts; ONO (3.82%) having light green color were arranged and further valued by performing phytochemical analysis of plant extracts. The highest quantity of phenolic content was observed in Ethanolic, Chloroform, and n-hexane extract of fruit with values 97.0 ± 0.91, 39.5 ± 0.55, and 8.1 ± 0.67 μg GAE/mg extract respectively.
Conclusion:
It very well might be deduced from this research that in-vitro investigation of the O. bracteatum plant showed a critical zone of inhibition against Escherichia coli, Bacillus subtilis, Staph aureus, klebsiella pneumonia, and Pseudomonas aeruginosa.
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