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Published on: September 17, 2021
Adhesion Study of Pseudomonas aeruginosa on Different Urinary Catheter Materials
Yogesh B Bele1, Prashant V Thakare2, Niraj A Ghanwate1
1Department of Microbiology, Sant Gadge Baba Amravati University, Amravati, Maharashtra, 444602, India.
Background:
Foley catheters, composed of various materials, can affect bacterial contamination, adhesion, and subsequent biofilm formation. Biofilm formation by Pseudomonas aeruginosa is a major virulence factor. As catheterization duration increases, so does the risk of contamination and biofilm adhesion. The aim of this study was to investigate bacterial adherence to different Foley urinary catheter materials.
Methods:
A total of 300 used urinary catheters were analyzed for bacterial contamination. The bacteria isolated from these catheters were studied for biofilm formation using the tissue culture plate method. Sections of new Foley catheters made of latex, polyvinyl chloride (PVC), and silicone were exposed to a high biofilm-forming strain of P. aeruginosa to determine which material was more susceptible to bacterial attachment and biofilm formation. The surface morphology of the catheter materials was analyzed using scanning electron microscopy (SEM).
Results:
Of the 300 urinary catheters, 270 were contaminated with various uropathogens. A latex Foley catheter became contaminated with P. aeruginosa at 72 hours, while PVC and silicone catheters were infected at 120 hours. SEM analysis revealed biofilm formation on the catheter surfaces.
Conclusions:
Among the contaminated catheters, 49% contained P. aeruginosa, forming a consistent biofilm. Latex catheters were more susceptible to early infection compared to PVC and silicone.
Insights
Latex Foley catheters show earlier bacterial contamination and biofilm formation compared to PVC and silicone. This highlights material differences in preventing Pseudomonas aeruginosa infections during urinary catheterization.
Area of Science:
- Urology
- Infectious Diseases
- Biomaterials Science
Background:
- Foley catheter material influences bacterial contamination, adhesion, and biofilm formation.
- Pseudomonas aeruginosa biofilm formation is a significant virulence factor in catheter-associated urinary tract infections.
- Increased catheterization duration correlates with higher contamination and biofilm adhesion risks.
Purpose of the Study:
- To investigate bacterial adherence to different Foley urinary catheter materials.
- To compare the susceptibility of latex, PVC, and silicone Foley catheters to bacterial attachment and biofilm formation.
Main Methods:
- Analysis of 300 used urinary catheters for bacterial contamination.
- Assessment of biofilm formation using the tissue culture plate method.
- Exposure of new catheter materials (latex, PVC, silicone) to P. aeruginosa and SEM analysis for surface morphology and biofilm.
Main Results:
- 270 out of 300 catheters were contaminated; 49% contained P. aeruginosa with consistent biofilm.
- Latex Foley catheters showed P. aeruginosa contamination at 72 hours.
- PVC and silicone catheters were infected at 120 hours, with SEM confirming biofilm formation.
Conclusions:
- Latex Foley catheters are more susceptible to early P. aeruginosa infection and biofilm formation than PVC and silicone.
- Material composition significantly impacts bacterial adherence and biofilm development on urinary catheters.
- Findings suggest material choice may influence the risk of catheter-associated infections.
