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Related Concept Videos

In-situ Hybridization02:31

In-situ Hybridization

9.2K
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
9.2K

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Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization
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Visualization of Microbiota in Tick Guts by Whole-mount In Situ Hybridization

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RNA In Situ Hybridization to Visualize Tick-Borne Viruses.

Allen T Esterly1, Erin S Reynolds2,3,4, Charles E Hart5

  • 1Section of Infectious Diseases, Department of Internal Medicine, Yale School of Medicine, New Haven, CT, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 13, 2025
PubMed
Summary
This summary is machine-generated.

This study details a new RNA in situ hybridization (ISH) method for visualizing Powassan virus (POWV) RNA in infected tissues. The technique uses a commercial kit for precise detection of viral RNA within cellular architecture.

Keywords:
BrainHistologyPOWVRNA in situ hybridization

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Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining
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Area of Science:

  • Virology
  • Pathobiology
  • Molecular Biology

Background:

  • Understanding tick-borne virus infection requires visualizing tissue and cellular architecture.
  • RNA in situ hybridization (ISH) is a key technique for detecting RNA targets within tissues.

Purpose of the Study:

  • To describe an RNA ISH methodology for detecting Powassan virus (POWV) RNA.
  • To enable visualization of viral RNA within infected tissue sections.

Main Methods:

  • Utilized a commercially available RNA ISH kit.
  • Employed specific oligonucleotide probes to detect complementary RNA transcripts.
  • Applied chromogenic or fluorescent staining for visualization.

Main Results:

  • Successfully hybridized specific probes to POWV RNA within infected tissues.
  • Demonstrated the capability to visualize viral RNA localization.

Conclusions:

  • The described RNA ISH methodology provides a reliable approach for studying POWV infection.
  • This technique aids in understanding the pathobiology of tick-borne virus infections at the cellular level.