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<i>Ixodes scapularis</i> STING promotes Powassan virus infection in ticks.

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Virus Propagation and Cell-Based Colorimetric Quantification
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Quantification of Tick-Borne Flaviviruses Using Focus Forming Assays.

Allen T Esterly1, Erin S Reynolds2,3,4, Saravanan Thangamani5,6

  • 1Section of Infectious Diseases, Department of Internal Medicine, Yale School of Medicine, New Haven, CT, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 13, 2025
PubMed
Summary
This summary is machine-generated.

Accurate quantification of Powassan virus (POWV) and Deer tick virus (DTV) is crucial for understanding these tick-borne flaviviruses. This study presents a repeatable methodology for quantifying POWV or DTV in a Biosafety Level 3 setting.

Keywords:
Deer tick virusPowassan virusTick-borne diseaseTick-borne virusViral quantification

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Area of Science:

  • Virology
  • Public Health
  • Arthropod-borne Viruses

Background:

  • Tick-borne flaviviruses, including Powassan virus (POWV), represent significant public health threats.
  • POWV and its lineage Deer tick virus (DTV) are emerging North American arboviruses causing neurotropic disease.
  • Accurate quantification of POWV/DTV is essential for research into their biology, pathogenesis, and transmission.

Purpose of the Study:

  • To develop and describe a repeatable methodology for quantifying POWV or DTV.
  • To enable accurate measurement of these viruses within a Biosafety Level 3 (BSL-3) environment.

Main Methods:

  • Implementation of a standardized quantification process for POWV/DTV.
  • Utilizing a Biosafety Level 3 (BSL-3) laboratory setting for safe handling and analysis.
  • Focus on establishing a repeatable methodology for consistent results.

Main Results:

  • A defined process for quantifying POWV or DTV has been established.
  • The methodology is designed for repeatability and accuracy within a BSL-3 setting.
  • This work facilitates further research on these significant tick-borne viruses.

Conclusions:

  • The described methodology provides a reliable means to quantify POWV and DTV.
  • This advancement supports critical research on tick-borne flaviviruses impacting human health.
  • Accurate quantification is key to understanding and combating POWV/DTV infections.