Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

  1. Home
  3. Research Domains
  5. Environmental Sciences
  7. Pollution And Contamination
  9. Environmental Biogeochemistry
  11. Human Biomonitoring Of Urinary Neonicotinoids And Their Metabolites By Ultra-high Performance Liquid Chromatography Tandem With Mass Spectrometry And Their Association With Oxidative Stress

Human biomonitoring of urinary neonicotinoids and their metabolites by ultra-high performance liquid chromatography tandem with mass spectrometry and their association with oxidative stress

Fenfang Deng1, Xiangyu Jia1, Rongfei Peng1

  • 1Institute of Public Health, Guangzhou Medical University & Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China.

Journal of Pharmaceutical and Biomedical Analysis
|June 13, 2025

Related Experiment Videos

Ecotoxicological Methodologies to Evaluate Biomarkers at Different Scales in Neotropical Anurans
08:14

Ecotoxicological Methodologies to Evaluate Biomarkers at Different Scales in Neotropical Anurans

Published on: April 28, 2023

347
Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications
08:41

Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications

Published on: July 14, 2017

9.3K
Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
09:33

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors

Published on: February 7, 2018

7.4K

View abstract on PubMed

Summary
This summary is machine-generated.

Human biomonitoring reveals neonicotinoid exposure linked to oxidative stress. This study developed a reliable method to measure urinary neonicotinoids, finding thiacloprid significantly elevates oxidative stress markers.

Area of Science:

  • Environmental Chemistry
  • Toxicology
  • Analytical Chemistry

Background:

  • Neonicotinoids are widely used pesticides with potential adverse health effects.
  • Human biomonitoring of urinary neonicotinoids is crucial for assessing population exposure and health risks.

Purpose of the Study:

  • To develop and validate an automated method for determining urinary neonicotinoids and metabolites.
  • To assess human exposure levels and their association with oxidative stress biomarkers.

Main Methods:

  • Automated liquid-liquid extraction and ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS).
  • Method validation for linearity, recovery, and precision.
  • Bayesian Kernel Machine Regression analysis to assess associations with oxidative stress biomarkers.
Keywords:
Human BiomonitoringNeonicotinoid InsecticidesOxidative StressSample Preparation

Related Experiment Videos

Ecotoxicological Methodologies to Evaluate Biomarkers at Different Scales in Neotropical Anurans
08:14

Ecotoxicological Methodologies to Evaluate Biomarkers at Different Scales in Neotropical Anurans

Published on: April 28, 2023

347
Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications
08:41

Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications

Published on: July 14, 2017

9.3K
Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
09:33

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors

Published on: February 7, 2018

7.4K

Main Results:

  • The validated method demonstrated excellent analytical performance (linearity, recovery, precision).
  • Significant differences in urinary neonicotinoid concentrations were observed between males and females.
  • Positive associations were found between urinary neonicotinoids and oxidative stress biomarkers, with thiacloprid being a key contributor.

Conclusions:

  • The developed method is efficient and reliable for large-scale human biomonitoring of neonicotinoids.
  • Neonicotinoid exposure is associated with increased oxidative stress in the human population.
  • Urinary neonicotinoid levels and their health implications warrant further investigation.
Urinary Metabolites