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In Vitro Fertilization01:24

In Vitro Fertilization

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In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...
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  11. Effect Of Fgf2, Lif, Igf1 Supplementation On Pregnancy Success Following Embryo Transfer Of In Vitro Derived Embryos

Effect of FGF2, LIF, IGF1 supplementation on pregnancy success following embryo transfer of in vitro derived embryos

K S McDonald1, J N Drum1, J G N Moraes2

  • 1Division of Animal Sciences, University of Missouri, Columbia, MO, USA.

Theriogenology
|June 13, 2025

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View abstract on PubMed

Summary
This summary is machine-generated.

Supplementing bovine embryo culture with growth factors FGF2, LIF, and IGF1 (FLI) improved placental development. FLI supplementation shows promise for enhancing in vitro embryo production and pregnancy success in cattle.

Area of Science:

  • * Bovine reproductive biology
  • * Embryology
  • * Developmental biology

Background:

  • * In vitro embryo production (IVP) systems aim to improve embryo competence.
  • * Previous studies showed that adding FGF2, LIF, and IGF1 (FLI) to culture medium enhanced bovine embryo development and cryosurvival.
  • * Transcriptomic analysis indicated FLI influences conceptus/placental development and maternal-embryo interactions, despite similar Day 15 phenotypes.

Purpose of the Study:

  • * To investigate the impact of FLI supplementation during in vitro culture on the pregnancy establishment ability of bovine embryos after slow-rate freezing.
  • * To assess if FLI influences placental development and maternal-embryo crosstalk in vivo.

Main Methods:

  • * In vitro produced bovine blastocysts were cultured with or without FLI.
Keywords:
CryopreservationEmbryo transferFGF2IGF1

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  • * Grade 1 embryos (6-1 or 5-1) were cryopreserved using slow-rate freezing.
  • * Single frozen-thawed embryos were transferred to 192 synchronized recipient females across three locations.
  • * Blood samples were collected on Days 7, 19, 20, and 24 for hormone and gene expression analysis.
  • * Pregnancy diagnosis was performed via transrectal ultrasonography after Day 28.

    • Main Results:

    • * Pregnancy rates per ET were similar between FLI-treated and control groups across all locations (P = 0.3).
    • * Progesterone levels and interferon-stimulated gene (ISG15, MX2, OAS1) expression on Days 7, 19, 20, and 24 were not significantly different between groups (P > 0.05).
    • * Recipients of FLI-treated embryos showed significantly increased circulating concentrations of pregnancy-associated glycoproteins (PAGs) (P = 0.0061), indicating advanced placental development.

    Conclusions:

    • * FLI supplementation did not negatively impact early embryo development or pregnancy establishment rates in this study.
    • * Increased PAG concentrations suggest FLI enhances placental development and potentially improves embryo viability beyond Day 30.
    • * FLI supplementation is a promising strategy to improve bovine IVP systems and pregnancy outcomes.
    In vitro fertilization
    LIF