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Updated: Jun 16, 2025
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Dye-decolorising peroxidase DyP1B from Pseudomonas fluorescens: Expression, reconstitution and reaction with
Rahman Rahmanpour1, Timothy D H Bugg1
1Department of Chemistry, University of Warwick, Coventry, United Kingdom.
Abstract:
Several bacterial dye-decolorising peroxidases have been identified, that have activity for oxidation of lignin model compounds and polymeric lignin. This article describes biochemical methods for lignin-oxidising peroxidase DyP1B from Pseudomonas fluorescens Pf-5. The article presents methods for: (1) enzyme purification and heme reconstitution; (2) steady-state kinetic enzyme assays; (3) pre-steady state kinetic analysis; (4) testing of the enzyme against polymeric lignin substrates.
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