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Updated: Jun 16, 2025

Maintaining Biological Cultures and Measuring Gene Expression in Aphis nerii: A Non-model System for Plant-insect Interactions
Published on: August 31, 2018
Chromosome-level genome assembly of the aphid Megoura crassicauda
Tianyu Huang1,2, Xu Cheng2, Bing Wang3
1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Abstract:
Megoura crassicauda (Hemiptera: Aphididae) is a major pest species that inflicts significant damage on various legume crops worldwide, causing substantial global economic losses. In this study, we present a chromosome-scale genome assembly of M. crassicauda. By integrating PacBio long-read sequencing, Illumina short-read sequencing, and Hi-C scaffolding techniques, we constructed a genome assembly spanning 424.45 Mb genome. Approximately 93.74% of the assembly was successfully anchored into five scaffolds, with contig and scaffold N50 reaching 5.55 Mb and 103.55 Mb, respectively. The genome completeness, as evaluated by BUSCO, achieved a completeness score of 97.75%. Additionally, a total of 14,717 protein-coding genes were identified. This high-quality genome assembly of M. crassicauda serves as a valuable genomic resource, facilitating further studies into the ecological adaptations of M. crassicauda and the development of pest control strategies.
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