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  11. Human Glioblastoma (u87) Cells Grown In 3d Culture Showed A Radio-resistance To X-ray And Proton Radiation

Human glioblastoma (U87) cells grown in 3D culture showed a radio-resistance to X-ray and proton radiation

Dea A Kartini1, Pharewa Karoon1, Yuwadee Malad1

  • 1School of Physics, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand.

Radiological Physics and Technology
|June 13, 2025

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View abstract on PubMed

Summary
This summary is machine-generated.

This study found that U87 glioma cells in 3D culture are more resistant to radiation but show higher sensitivity to proton therapy than X-rays, with a relative biological effectiveness (RBE) of 1.15.

Area of Science:

  • Radiation Oncology
  • Cell Biology
  • Biophysics

Background:

  • Glioblastoma multiforme is a highly malignant brain tumor resistant to conventional radiotherapy.
  • Proton radiotherapy offers precise dose delivery to deep-seated tumors, minimizing damage to surrounding tissues.
  • Investigating cellular response in 3D cultures mimics in vivo conditions more closely than traditional 2D models.

Purpose of the Study:

  • To compare the radiobiological response of human glioma (U87) cells in 3D culture to X-ray and proton irradiation.
  • To evaluate the influence of 3D culture environment on cell survival and radiosensitivity.
  • To determine the relative biological effectiveness (RBE) of proton beams compared to X-rays for U87 cells in both 2D and 3D cultures.

Main Methods:

  • U87 human glioma cells were cultured in a 3D bio-phantom using Matrigel.
Keywords:
3D cell cultureGlioblastomaProton radiotherapySurvival curve

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  • Cells were irradiated with 6 MV X-rays and 70 MeV monoenergetic proton beams.
  • Cell survival was assessed using colony formation assays, and survival curves were compared between 2D and 3D cultures.

    • Main Results:

    • U87 cells in 3D culture exhibited increased radio-resistance in the high-dose region compared to 2D cultures.
    • U87 cells demonstrated higher sensitivity to proton irradiation than X-ray irradiation in both 2D and 3D culture setups.
    • The RBE10% values were 1.15 for 3D cultures and 1.29 for 2D cultures.

    Conclusions:

    • U87 cells in the developed 3D culture system display radio-resistant characteristics.
    • Proton irradiation shows enhanced efficacy over X-rays for U87 glioma cells, irrespective of the culture dimensionality.
    • The findings support the potential of proton therapy for glioblastoma treatment, particularly in overcoming radioresistance.