Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

1.8K
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
1.8K
Differential Staining Technique01:26

Differential Staining Technique

2.7K
Differential staining is an essential microbiological technique that exploits variations in cell wall structures to classify and identify microorganisms. It facilitates the distinction of bacteria, aiding in diagnostic and research applications. Two of the most widely used differential staining methods are Gram staining and acid-fast staining, both of which rely on the chemical and structural differences in bacterial cell walls.Gram Staining TechniqueGram staining differentiates bacteria by...
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Light-controlled microgripper punches above its weight.

Nature·2026
Same author

Lattice, Grain, and Texture: Opportunities for Performance Enhancement of Layered Oxide Cathodes Informed by LiCoO<sub>2</sub>.

Chemical reviews·2026
Same author

Phonon-polaritonic skyrmions: transition from bubble- to Néel-type.

Light, science & applications·2026
Same author

Tunable polaritonic topologies generated by non-local photonic modes.

Nature nanotechnology·2026
Same author

Tunable Skyrmion, Meron, and Skyrmion Bag Textures in Surface Phonon Polariton Lattices.

Nanophotonics (Berlin, Germany)·2026
Same author

Polarization-Dependent Elliptical and Rectangular Mie Voids.

Small (Weinheim an der Bergstrasse, Germany)·2026

Related Experiment Video

Updated: May 6, 2026

Soft Lithographic Functionalization and Patterning Oxide-free Silicon and Germanium
12:38

Soft Lithographic Functionalization and Patterning Oxide-free Silicon and Germanium

Published on: December 16, 2011

14.7K

Diffractive microoptics in porous silicon oxide by grayscale lithography.

Leander Siegle, Dajie Xie, Corey A Richards

    Optics Express
    |June 14, 2025
    PubMed
    Summary

    We developed 3D printed diffractive microoptics using two-photon polymerization grayscale lithography (2GL) in porous silicon oxide. This novel method enhances fabrication efficiency and optical performance for advanced micro-devices.

    More Related Videos

    Patterning via Optical Saturable Transitions - Fabrication and Characterization
    08:19

    Patterning via Optical Saturable Transitions - Fabrication and Characterization

    Published on: December 11, 2014

    6.8K
    Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging
    07:14

    Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging

    Published on: April 11, 2025

    440

    Related Experiment Videos

    Last Updated: May 6, 2026

    Soft Lithographic Functionalization and Patterning Oxide-free Silicon and Germanium
    12:38

    Soft Lithographic Functionalization and Patterning Oxide-free Silicon and Germanium

    Published on: December 16, 2011

    14.7K
    Patterning via Optical Saturable Transitions - Fabrication and Characterization
    08:19

    Patterning via Optical Saturable Transitions - Fabrication and Characterization

    Published on: December 11, 2014

    6.8K
    Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging
    07:14

    Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging

    Published on: April 11, 2025

    440

    Area of Science:

    • Optics and Photonics
    • Materials Science
    • Additive Manufacturing

    Background:

    • Traditional microoptic fabrication often requires complex support structures, increasing time and limiting design.
    • Two-photon polymerization (TPP) is a common microfabrication technique, but can be limited in speed and accuracy.
    • Porous materials offer unique integration possibilities for micro-optics.

    Purpose of the Study:

    • To demonstrate focusing and imaging capabilities of diffractive microoptics fabricated using 2GL within porous silicon oxide.
    • To showcase the advantages of 2GL over standard TPP, including improved shape accuracy and throughput.
    • To present a novel fabrication approach that integrates microoptics directly into a supporting porous matrix.

    Main Methods:

    • Diffractive microoptics were fabricated using two-photon polymerization grayscale lithography (2GL) within a porous silicon oxide (SiO2) matrix.
    • Singlet diffractive lenses (500 µm diameter, NA up to 0.6) were created and characterized by measuring focal plane and optical axis intensity distributions.
    • A doublet lens system (600 µm diameter, <60 µm thickness) was designed, fabricated, and tested for imaging performance using a USAF 1951 resolution test chart.

    Main Results:

    • The 2GL process enabled high shape accuracy and increased fabrication throughput compared to standard TPP.
    • Singlet lenses demonstrated effective focusing capabilities.
    • The doublet lens system achieved a resolution of 287 line pairs per millimeter (lp/mm), validating its imaging performance.

    Conclusions:

    • 3D printing diffractive microoptics into porous SiO2 using 2GL offers a promising, integrated solution.
    • This technique reduces fabrication time, design constraints, and increases the optically active area.
    • The method holds potential for creating complex and unconventional microoptical systems.