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Milliseconds photon-to-photon latency projection system for adaptive optogenetics applications
Optics Express
|June 14, 2025
Summary
We developed a novel projection method for microscopy, enabling dynamic projection mapping with millisecond latency. This system accurately targets moving cells for optogenetic stimulation, overcoming previous limitations in live-cell research.
Area of Science:
- Biotechnology
- Microscopy
- Optogenetics
Background:
- Dynamic projection mapping is essential for adapting projections to moving objects.
- Microscopy requires similar dynamic projection for stimulating photosensitive cells in motion.
Purpose of the Study:
- To propose a novel projection method for microscopy with millisecond latency.
- To enable dynamic projection mapping for optogenetic stimulation of moving cells.
Main Methods:
- Utilized a low-latency digital micromirror device.
- Integrated a high-speed vision system for real-time tracking.
- Developed a system for dynamic light projection responding to object movement and deformation.
Main Results:
- Achieved a photon-to-photon latency of 6.56 ± 1.76 ms.
- Successfully demonstrated dynamic projection mapping on a randomly moving target.
- Enabled precise light projection for stimulating moving cells.
Conclusions:
- The developed system provides millisecond-latency projection for microscopy.
- This technique is suitable for dynamic optogenetic stimulation of moving or deforming biological samples.
- Advances dynamic projection mapping applications in live-cell imaging and manipulation.
