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Updated: Apr 23, 2026

Protocols for Robust Herbicide Resistance Testing in Different Weed Species
Published on: July 2, 2015
Development of a nanoemulsion bioherbicide and its herbicidal efficacy
Mahmudul Hasan1, Anis Syahirah Mokhtar2, Mst Motmainna1,3
1Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia.
Background:
Bioherbicide applications are environmental-friendly approaches to control weeds toward sustainable agriculture. Hence, the current research focused on developing a ready-to-use nanoemulsion bioherbicide from the current less economical WeedLock bioherbicide, furthermore evaluating its weed control efficacy. Nanoemulsion composition comprised 2-undecanone (active ingredient), Termul 5030, POE(20)SMO, and water. The selected formulations were incorporated with 5% active ingredient.
Results:
Among the 11 formulations tested, three formulations (WLRF7, WLRF9, and WLRF11) showed remarkable stability, in centrifugation, storage stability, and thermostability. Furthermore, screening for weed control efficacy on Ageratum conyzoides L., Eleusine indica (L.) Gaertn. and Cyperus iria L. at 1345.50 L ha-1 (WeedLock recommended rate) WLRF9 formulation exhibited a greater efficacy of 98.39% than WLRF7 (83.90%) and WLRF11 (91.31%). In the dose-response experiment, at the recommended rate, 98.16%, 98.25%, and 98.38% control efficacy were produced by the WLRF9 formulation, glufosinate-ammonium, and glyphosate isopropyl-amine, respectively.
Conclusion:
Evidently, the new bioherbicide formulation (WLRF9) has shown excellent efficacy and potential to control weeds. © 2025 Society of Chemical Industry.
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