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  11. Metabolomics Analysis Reveals That Anoectochilus Roxburghii Extract Enhances Immunity In Immunosuppressed Rats By Modulating Lipid Metabolism And Amino Acid Metabolism

Metabolomics Analysis Reveals That Anoectochilus roxburghii Extract Enhances Immunity in Immunosuppressed Rats by Modulating Lipid Metabolism and Amino Acid Metabolism

Xu Fang1,2, Ying Yang3, Xinmei Cao3

  • 1Department of Stomatology, Tongde Hospital of Zhejiang Province, Hangzhou, China.

Biomedical Chromatography : BMC
|June 14, 2025

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View abstract on PubMed

Summary
This summary is machine-generated.

Anoectochilus roxburghii extract boosts immunity in immunosuppressed rats by restoring organ health and normalizing key immune markers. This study reveals its therapeutic effects are linked to modulating lipid and amino acid metabolism.

Area of Science:

  • Immunology
  • Metabolomics
  • Pharmacology

Background:

  • Anoectochilus roxburghii (AR) is known for immunomodulatory effects, but mechanisms are unclear.
  • Cyclophosphamide (CTX) induces immunosuppression, making it a relevant model for studying immune enhancement.
  • Understanding AR's impact on metabolic pathways is crucial for elucidating its therapeutic actions.

Purpose of the Study:

  • To investigate the metabolomic changes and therapeutic effects of AR in CTX-induced immunosuppressed rats.
  • To identify potential biomarkers associated with AR's immunomodulatory activity.
  • To elucidate the metabolic pathways involved in AR's immune-enhancing effects.

Main Methods:

  • Establishment of an immunosuppressed rat model using cyclophosphamide (CTX).
  • Gas chromatography-mass spectrometry (GC-MS) based metabolomics analysis.
Keywords:
Anoectochilus roxburghiiamino acid metabolismimmunosuppressionlipid metabolism

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  • Histopathological and biochemical analyses, including spleen and thymus indices, and cytokine levels (TNF-α, IL-2, IL-10).

    • Main Results:

    • AR treatment significantly restored spleen and thymus indices in immunosuppressed rats.
    • AR normalized elevated levels of TNF-α and IL-2, and increased decreased IL-10 levels.
    • Metabolomic analysis identified differential metabolites, with lactic acid, threonine, and palmitic acid positively correlated with immune organ indices.

    Conclusions:

    • Anoectochilus roxburghii extract demonstrates significant immunomodulatory effects in immunosuppressed rats.
    • AR enhances immunity by restoring lymphoid organ indices and balancing pro-inflammatory and anti-inflammatory cytokine levels.
    • The therapeutic benefits of AR are associated with the modulation of lipid and amino acid metabolism.
    metabolome