JoVE - Journal of Visualized Experiments
JoVE Visualize
Contact Us

LMNB1 regulates breast cancer cell senescence and migration through PPAR signaling pathway

  • 0Department of Breast and Thyroid Surgery, The First People's Hospital of Yunnan Province, 157 Jinbi Road, Xishan District, Kunming, 650032, Yunnan, China.
Discover oncology +

|

June 14, 2025

Abstract

Breast cancer is the malignant tumor with the highest incidence in the world. LMNB1 is an important component of the nuclear backbone. LMNB1 is abnormally highly expressed in breast cancer, but its role in tumors still needs to be comprehensively studied. In this study, we overexpress and knockdown LMNB1 in breast cancer cells and explore the effects of LMNB1 on cell proliferation, senescence, migration, and underlying mechanisms. We analyzed the expression level of LMNB1 in breast cancer and its clinical relevance utilizing bioinformatics methods, qRT-PCR, and western blot. To elucidate the effects and mechanisms of LMNB1 on breast cancer cells, we performed SA-β-gal staining, CCK-8, colony formation, wound healing, and Transwell assays in breast cancer cells with LMNB1 overexpression and knockdown, and then examined the expression of relevant genes and proteins. In addition, we also analyzed the signaling pathways regulated by LMNB1 with the help of bioinformatics tools. Overexpression of LMNB1 significantly inhibited the senescence of cancer cells and promoted cell proliferation, migration, and invasion. The opposite results were obtained in LMNB1 knockdown cells. LMNB1 inhibited the PPAR signaling pathway in breast cancer. Our study suggests that LMNB1 is a novel therapeutic target in breast cancer.

Related Concept Videos

Abnormal Proliferation 02:23

4.5K

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

Interactions Between Signaling Pathways 01:19

6.2K

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...

The Intrinsic Apoptotic Pathway 01:31

6.4K

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...

Negative Regulator Molecules 01:23

35.2K

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.

Three of the best-understood negative regulators are p53, p21, and retinoblastoma protein (Rb). The regulatory roles of each of these proteins were discovered after faulty copies were found in cells with uncontrolled replication (i.e.,...

mTOR Signaling and Cancer Progression 03:03

3.8K

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

Canonical Wnt Signaling Pathway 02:54

8.7K

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...