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Faecal amino acids stability: investigating optimal sampling conditions for analysis
Roza C M Opperman1,2,3, Eva Vermeer4,5,6, Sofie Bosch7,8
1Department of Gastroenterology and Hepatology, Amsterdam University Medical Centre, Vrije Universiteit Amsterdam, Amsterdam, 1081 HV, The Netherlands. r.opperman@amsterdamumc.nl.
Introduction:
Faecal amino acids are promising non-invasive diagnostic biomarkers, but stability remains unclear.
Objectives:
This study examined whether storage conditions, sampling site within the sample, freeze-thaw cycles, and the OMNImet®·GUT device affect faecal amino acid concentrations.
Methods:
Faecal samples from three donors underwent various pre-analytical conditions. Amino acids were analysed through targeted liquid chromatography-tandem mass spectrometry.
Results:
Most amino acids remained stable across sampling sites and freeze-thaw cycles. Storage at -20 °C preserved integrity, whereas 4 °C and 20 °C storage led to variations. The OMNImet®·GUT device stabilised some amino acids but showed inconsistencies.
Conclusion:
Pre-analytical conditions influence faecal amino acid concentrations. Standardisation is essential for biomarker reliability.
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