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Published on: January 20, 2017
High-Throughput Yeast Two-Hybrid Technology for Investigating Molecular Virology
Sisi Xia1,2, Jialin Wang3, Xiaoping Huo3
1Department of Biological Engineering, Wuhan Polytechnic University, Wuhan, China.
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High-throughput yeast two-hybrid (YTH) screening systems are essential technologies for understanding the viral life cycle, pathogenesis, and development of antiviral therapies. They are commonly used in molecular virology to identify and analyze the complex molecular interactions between viruses and host cells. This chapter details the relevant information and experimental procedures for systematically screening and identifying interactions between viral and host proteins via high-throughput YTH technology.

