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Related Experiment Video

Updated: Jun 16, 2025

Using Zebrafish Models of Human Influenza A Virus Infections to Screen Antiviral Drugs and Characterize Host Immune Cell Responses
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Zebrafish as a Model for Studying Virus-Host Interactions.

Jing Wang1,2, Chunfu Zheng2, Wuhan Xiao3,4

  • 1Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of Sciences, Hubei Hongshan Laboratory, Wuhan, China.

Methods in Molecular Biology (Clifton, N.J.)
|June 14, 2025
PubMed
Summary

Zebrafish (Danio rerio) offer a powerful vertebrate model for studying virus-host interactions. This chapter details experimental protocols for analyzing viral infections and host responses using zebrafish, including gene editing and molecular analysis.

Keywords:
In vivo modelSVCVVirus–host interactionZebrafish

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Area of Science:

  • Virology
  • Immunology
  • Genetics
  • Zebrafish Model Organisms

Background:

  • Viruses and hosts engage in a continuous evolutionary arms race, driving viral clearance and immune escape mechanisms.
  • Understanding virus-host interactions is crucial for developing effective antiviral strategies.
  • Zebrafish (Danio rerio) are emerging as a valuable vertebrate model for studying these complex interactions due to their advantageous biological and genetic characteristics.

Purpose of the Study:

  • To provide detailed experimental protocols for analyzing virus-host interactions in a zebrafish model.
  • To demonstrate the application of these protocols using spring viremia of carp virus (SVCV) as a case study.
  • To establish a comprehensive reference for researchers investigating virus-host dynamics in zebrafish.

Main Methods:

  • CRISPR/Cas9 gene editing for generating knockout zebrafish.
  • Viral infection protocols for zebrafish larvae and adults.
  • Quantitative reverse transcription PCR (qRT-PCR) and Western blotting for gene and protein expression analysis.
  • Immunoprecipitation assays to detect host-viral protein interactions.
  • In vivo analysis of host gene resistance to viral infection.

Main Results:

  • Established comprehensive protocols for zebrafish-based virus-host interaction studies.
  • Demonstrated the utility of zebrafish for analyzing viral infection dynamics and host responses.
  • Provided methods for genetic manipulation and molecular analysis within the zebrafish model.
  • Successfully applied these methods to study spring viremia of carp virus (SVCV) interactions.

Conclusions:

  • Zebrafish provide a versatile and powerful model system for dissecting complex virus-host interactions.
  • The detailed protocols presented serve as an effective guide for researchers in the field.
  • This approach facilitates the study of viral pathogenesis, host immunity, and potential therapeutic targets.