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System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
Published on: April 5, 2017
Identifying Inhibitor Targets in Mycobacteria by Activity-Based Probe Profiling
Neetika Jaisinghani1, Isabel Sakarin2, Hiren V Patel1
1Department of Pharmacological Sciences, Stony Brook University, Stony Brook, NY, USA.
Abstract:
Activity-based protein profiling (ABPP) enables the detection of protein reactivity across entire proteomes. Here, we describe the application of ABPP to the identification of serine hydrolase inhibitor targets in Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis in humans. We highlight the adaptation of stable isotope labeling of amino acids (SILAC) to Mtb using a modified growth medium with an isotopically labeled sole nitrogen source and detail the resulting special requirements for proteomics analysis.
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