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Related Experiment Video

Updated: Jul 9, 2026

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis

Published on: April 5, 2017

Identifying Inhibitor Targets in Mycobacteria by Activity-Based Probe Profiling.

Neetika Jaisinghani¹, Isabel Sakarin², Hiren V Patel¹

¹Department of Pharmacological Sciences, Stony Brook University, Stony Brook, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)

|June 14, 2025

Summary

This summary is machine-generated.

Activity-based protein profiling identified serine hydrolase targets in Mycobacterium tuberculosis. This study adapted stable isotope labeling of amino acids in Mtb for improved proteomics analysis.

Keywords:

Activity-based protein profiling Inhibitor Mycobacteria Proteomics SILAC Tuberculosis

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Published on: November 22, 2024

Area of Science:

Biochemistry
Proteomics
Microbiology

Background:

Activity-based protein profiling (ABPP) is a powerful technique for studying protein function.
Mycobacterium tuberculosis (Mtb) causes tuberculosis, a significant global health concern.
Identifying Mtb-specific targets is crucial for developing new anti-tubercular therapies.

Purpose of the Study:

To apply ABPP for identifying serine hydrolase inhibitor targets in Mtb.
To adapt stable isotope labeling of amino acids (SILAC) for use in Mtb.
To optimize proteomics analysis for SILAC-labeled Mtb.

Main Methods:

Utilized ABPP with chemical probes to label active serine hydrolases in Mtb.
Adapted SILAC by using an isotopically labeled nitrogen source in a modified Mtb growth medium.
Developed specialized proteomics workflows to analyze SILAC-labeled Mtb samples.

Main Results:

Successfully identified several putative serine hydrolase targets in Mtb.
Demonstrated the feasibility of SILAC-based quantitative proteomics in Mtb.
Characterized the specific requirements for analyzing SILAC-labeled Mtb proteomes.

Conclusions:

ABPP is effective for discovering drug targets in Mtb.
SILAC-based proteomics can be successfully applied to Mtb research.
This methodology provides a foundation for further drug discovery efforts against Mtb.