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Updated: Jun 16, 2025

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

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Published on: March 24, 2012

Identification of Anticancer ROS Targets by Cysteine Reactivity Protein Profiling.

Junbing Zhang^1,2,3, Liron Bar-Peled^4,5,6

¹Krantz Family Center for Cancer Research, Massachusetts General Hospital Cancer Center, Charlestown, MA, USA. Junbingzhang@sinh.ac.cn.

Methods in Molecular Biology (Clifton, N.J.)

|June 14, 2025

Summary

This study details a chemoproteomic workflow to identify cysteine modifications. It helps understand how anticancer drugs induce reactive oxygen species (ROS) and affect cellular redox balance.

Keywords:

Anticancer drugs Chemical proteomics Cysteine Posttranslational modifications ROS

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Production and Detection of Reactive Oxygen Species ROS in Cancers

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Last Updated: Jun 16, 2025

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Production and Detection of Reactive Oxygen Species ROS in Cancers

Production and Detection of Reactive Oxygen Species ROS in Cancers

Published on: November 21, 2011

Area of Science:

Biochemistry
Proteomics
Chemical Biology

Background:

Cysteine reactivity profiling is a key chemoproteomic method for studying the cysteinome.
Cysteines are vital for cellular redox homeostasis, undergoing dynamic redox state changes.
Reactive oxygen species (ROS) play a role in cellular processes and disease states.

Purpose of the Study:

To present a detailed workflow for cysteine-based chemical proteomics.
To systematically identify cysteine modifications induced by anticancer drugs.
To investigate the impact of drug-induced ROS on the cysteinome.

Main Methods:

Development of a sample preparation workflow for cysteine-based chemical proteomics.
Utilizing chemical probes for covalent binding to reactive cysteines.
Quantitative analysis of cysteine abundance via mass spectrometry.

Main Results:

A robust workflow for identifying drug-induced cysteine modifications was established.
The method enables systematic profiling of the cysteinome under oxidative stress.
Insights into cysteine's role in anticancer drug-induced ROS signaling.

Conclusions:

The described workflow facilitates the study of cysteine reactivity in response to oxidative stress.
This approach aids in understanding the mechanisms of anticancer drugs.
It provides a foundation for further chemoproteomic investigations of redox biology.