In-situ Visualization of Level 3 LFPs Using a Lipophilic Red AIE Fluorescent Probe with a D-A Structure

  • 1Department of Critical Care Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, 1st Shuaifuyuan, Dongcheng District, Beijing, 100730, China.
  • 2Department of Forensic Science, Fujian Police College, Fuzhou, 350007, China. luliqin0118@163.com.
  • 3College of Materials Science and Engineering, Fuzhou University, Fujian, 350108, China.
  • 4Department of Forensic Science, Fujian Police College, Fuzhou, 350007, China. gx_zhuang@fjpsc.edu.cn.

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Abstract

Fingerprints are among the most commonly encountered forms of forensic evidence. However, current databases require relatively complete fingerprints to extract sufficient level 2 features for individual identification, thereby limiting the recognition of partial latent fingerprints (LFPs) collected from crime scenes. Therefore, visualizing level 3 features of LFPs (such as width, spacing, and distribution of ridge and pore) is of critical importance. In response to this challenge, this study developed a near-infrared AIE fluorescent probe (D-TPA-A-TXO) with a D-A structure. In ethanol/water binary solvents, D-TPA-A-TXO enabled rapid and distinct in-situ visualization of level 3 LFPs features across various substrates. Owing to its lipophilic nature, D-TPA-A-TXO rapidly dissolved from the solution into the lipid components of LFPs and continuously aggregated. Through the aggregation-induced emission (AIE) effect, high-resolution images of level 3 LFPs features could be directly captured by a standard camera. This advancement provides crucial technical support for enhancing the matching capability of partial fingerprints in future criminal investigations.

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