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Updated: Jun 16, 2025

Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography
Published on: January 17, 2020
The molecular landscape of cellular metal ion biology
Simran Kaur Aulakh1, Oliver Lemke2, Lukasz Szyrwiel3
1Molecular Biology of Metabolism Laboratory, The Francis Crick Institute, 1 Midland Road, NW1 1AT London, UK; Centre for Human Genetics, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Abstract:
Metal ions have crucial roles in cells, but the impact of their availability on biological networks is underexplored. We systematically quantified yeast cell growth and the corresponding metallomic, proteomic, and genetic responses to perturbations in metal availability along concentration gradients of all growth-essential metal ions. We report a remarkable metal concentration dependency of cellular networks, with around half of the proteome, and most signaling pathways such as target of rapamycin (TOR), being metal responsive. Although the biological response to each metal is distinct, our data reveal common properties of metal responsiveness, such as concentration interdependencies and metal homeostasis. Furthermore, our resource indicates that many understudied proteins have functions related to metal biology and reveals that metalloenzymes occupy central nodes in metabolic networks. This work provides a framework for understanding the critical role of metal ions in cellular function, with broader implications for manipulating metal homeostasis in biotechnology and medicine.
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