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  11. Coproduction Of Single Cell Protein And Pyrroloquinoline Quinone By Hyphomicrobium Denitrificans Using Molasses And Biogas Slurry

Coproduction of single cell protein and pyrroloquinoline quinone by Hyphomicrobium denitrificans using molasses and biogas slurry

Daoyang Zhu1, Qiuya Gu1, Xiaobin Yu1

  • 1Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Li-Hu Road, Wuxi, Jiangsu 214122, PR China.

Bioresource Technology
|June 14, 2025

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View abstract on PubMed

Summary
This summary is machine-generated.

This study efficiently coproduced single-cell protein (SCP) and pyrroloquinoline quinone (PQQ) using Hyphomicrobium denitrificans SSWJ1 from molasses and biogas slurry. This method offers a sustainable approach for alternative animal proteins and valuable wastewater products.

Area of Science:

  • Biotechnology
  • Microbial Fermentation
  • Sustainable Chemistry

Background:

  • Single-cell protein (SCP) and pyrroloquinoline quinone (PQQ) are valuable components in animal feed and functional foods.
  • Industrial waste streams like molasses and biogas slurry present opportunities for sustainable bioproduction.

Purpose of the Study:

  • To develop a method for the co-production of SCP and PQQ using Hyphomicrobium denitrificans SSWJ1.
  • To optimize fermentation conditions for maximizing SCP and PQQ yields from low-cost substrates.

Main Methods:

  • Evaluated the alkaline tolerance of H. denitrificans SSWJ1, identifying optimal growth at pH 8.
  • Compared SCP production in different media: molasses/biogas slurry vs. methanol/ammonium sulfate.
  • Implemented a two-stage oxygen delivery strategy in a 5-L fermenter.
Keywords:
Alternative proteinBioprocess optimizationFermentationMethylotrophic bacteria

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Main Results:

  • H. denitrificans SSWJ1 demonstrated optimal growth at pH 8.
  • SCP yields were higher in molasses and biogas slurry media (2.0 g/L) compared to methanol and ammonium sulfate media (0.7 g/L).
  • The optimized two-stage oxygen approach achieved 35.6 g/L SCP and 235.1 mg/L PQQ.

Conclusions:

  • A highly efficient strategy for coproducing SCP and PQQ from molasses and biogas slurry was established.
  • This process offers a sustainable route for producing alternative animal proteins.
  • The study highlights the potential for generating high-value products from wastewater resources.
Sustainable production
Waste valorization