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  11. Peo/gg Nanofiber-based Lipase Immobilization For Efficient Hydrolysis Of Selected Vegetable Oils And Industrial Biocatalysis

PEO/GG nanofiber-based lipase immobilization for efficient hydrolysis of selected vegetable oils and industrial biocatalysis

Ceyhun Işık1, Özgün Vatansever2, Sezer Eryılmaz1

  • 1Muğla Sıtkı Koçman University, Faculty of Science, Chemistry Department, Muğla 48000, Türkiye.

International Journal of Biological Macromolecules
|June 14, 2025

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View abstract on PubMed

Summary
This summary is machine-generated.

Lipase immobilized on poly(ethylene oxide)/guar gum nanofibers shows enhanced stability and reusability. The PEO4/GG nanofiber support offers superior performance for efficient biocatalysis in various applications.

Area of Science:

  • Biocatalysis and Enzyme Engineering
  • Materials Science and Nanotechnology

Background:

  • Enzyme immobilization on nanostructured supports enhances stability and reusability.
  • Electrospun nanofibers offer high surface area and porosity for effective enzyme loading.

Purpose of the Study:

  • To synthesize and characterize poly(ethylene oxide)/guar gum (PEO/GG) nanofibers for lipase immobilization.
  • To optimize immobilization parameters and evaluate the performance of lipase immobilized on PEO/GG nanofibers.

Main Methods:

  • Electrospinning of PEO/GG nanofibers with varying PEO concentrations.
  • Characterization of nanofiber porosity and surface area.
  • Optimization of lipase immobilization parameters (carrier amount, adsorption time, cross-linking, glutaraldehyde concentration).
  • Assessment of immobilized lipase stability (thermal, pH, storage, operational) and catalytic efficiency.
Keywords:
Lipase immobilizationNanofiberVegetable oil hydrolysis

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Main Results:

  • PEO4/GG nanofibers exhibited optimal porosity and surface area for enzyme immobilization.
  • Immobilized lipase demonstrated significantly improved thermal, pH, and storage stability compared to free lipase.
  • The immobilized enzyme showed high reusability and efficient hydrolysis of vegetable oils, especially polyunsaturated fatty acid-rich oils.

Conclusions:

  • PEO4/GG nanofibers serve as a robust and effective matrix for lipase immobilization.
  • The developed system shows great potential for industrial applications in hydrolytic biocatalysis, including food processing and environmental remediation.