Unveiling the antioxidant potential of new modified hyaluronan derivatives: Design, synthesis, characterization,...

Hyaluronan (HA), a glycosaminoglycan present in the extracellular matrix, serves as a structural component in various tissues and plays a crucial role in multiple biological processes. The network-forming ability and negative charge of HA significantly contribute to numerous biochemical activities in living organisms' tissues. By chemically modifying HA, several new biocompatible and degradable materials have been developed. The functionalization of the HA backbone was facilitated by the reactions between the hydroxyl groups in HA and the carbonyl groups of the corresponding reagents. In this study, novel aromatic derivatives of HA were formulated using 4-(dimethylamino)benzaldehyde and 2',4',6'-trihydroxyacetophenone. Characterization of the derivatives was performed through Fourier transform infrared spectroscopy and electronic spectrum analysis. Thermal stability was assessed using thermal gravimetric analysis and differential scanning calorimetry. The free-radical scavenging activities of the HA derivatives were evaluated using spectrophotometric ABTS and DPPH assays. Furthermore, molecular docking and pharmacokinetic investigations unveiled the antioxidant mechanisms and therapeutic applications of the HA derivatives. In conclusion, our findings demonstrate the potential of these two novel HA derivatives in the development of specialized wound dressings with anti-inflammatory and antioxidant properties. Additionally, these dressings could effectively combat pathogenic bacteria and aid in the treatment of chronic skin wounds.