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  9. Agricultural Molecular Engineering Of Nucleic Acids And Proteins
  11. Myricetin, As A Potential New Antiviral Agent Against Rana Grylio Ranavirus (rgrv)

Myricetin, as a potential new antiviral agent against Rana grylio ranavirus (RGRV)

Kun Peng1, Silu Tian2, Le Xu1

  • 1College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Sichuan Aquatic Animal Disease Control and Prevent Center, Chengdu, Sichuan 611130, China.

Fish & Shellfish Immunology
|June 14, 2025

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View abstract on PubMed

Summary
This summary is machine-generated.

Myricetin effectively combats Ranavirus (RGRV) in aquaculture, significantly reducing mortality in infected frogs. This natural compound inhibits viral replication and boosts host immunity, showing promise for disease control.

Area of Science:

  • Aquatic animal health
  • Virology
  • Natural product chemistry

Background:

  • Ranaviruses are highly lethal, threatening aquaculture survival.
  • Developing effective antiviral therapies is crucial for disease management.
  • Herbal bioactive compounds offer potential for preventing and controlling aquatic diseases.

Purpose of the Study:

  • To evaluate the in vitro antiviral activity of four flavonoids against Ranavirus (RGRV).
  • To investigate the mechanism of action of the most potent flavonoid.
  • To assess the in vivo efficacy of the most potent flavonoid in a fish model.

Main Methods:

  • In vitro screening of myricetin (MYR), luteolin (LUT), epigallocatechin gallate (EGCG), and rutin (RUT) against RGRV.
  • Analysis of MYR's effect on viral replication stages and gene expression (immediate-early and delayed-early genes).
Keywords:
Anti-inflammatoryAntivirusImmunopotentiationMyricetin

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  • Investigation of MYR's impact on host immune pathways (JAK-STAT and NF-κB signaling).
  • In vivo challenge assay using pig frogs (Rana grylio) infected with RGRV.

    • Main Results:

    • All four tested flavonoids exhibited anti-RGRV activity in vitro.
    • Myricetin (MYR) showed the most potent, dose-dependent antiviral effect.
    • MYR inhibited all RGRV infection stages, particularly early replication, by down-regulating viral gene expression.
    • MYR enhanced host immunity and reduced inflammation via JAK-STAT pathway upregulation and NF-κB pathway downregulation.
    • In vivo, MYR significantly reduced mortality in RGRV-infected pig frogs.

    Conclusions:

    • Myricetin demonstrates significant antiviral activity against Ranavirus (RGRV).
    • MYR effectively inhibits viral replication and modulates host immune responses.
    • Myricetin shows considerable promise as a therapeutic agent for Ranavirus infections in aquaculture.
    Ranaviruses