Jove
Visualize
Contact Us

Related Concept Videos

Embryonic Stem Cells00:57

Embryonic Stem Cells

3.4K
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
3.4K
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
  1. Home
  3. Effects Of An Environmental Chemical Mixture On Early-stage Embryo Development: In Vitro Evidence From Human Embryonic Stem Cells.
  1. Home
  3. Effects Of An Environmental Chemical Mixture On Early-stage Embryo Development: In Vitro Evidence From Human Embryonic Stem Cells.

Related Experiment Video

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation
17:28

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Published on: June 17, 2015

12.6K

Effects of an environmental chemical mixture on early-stage embryo development: In vitro evidence from human

Bai Li1, Yihang Kevin Pan1, Xiaolei Jin2

  • 1Department of Biology, University of Ottawa, Ottawa, Ontario, Canada.

Toxicology in Vitro : an International Journal Published in Association with BIBRA
|June 14, 2025

View abstract on PubMed

Summary
This summary is machine-generated.

Environmental chemical mixtures, like the Nunavik Chemical Mixture (NCM), harm early embryo development by affecting cell viability and gene expression. Understanding these mixture effects is crucial for assessing pregnancy risks.

Keywords:
Chemical mixtureDevelopmental toxicityEmbryonic stem cellEmbryotoxicityEnvironmental contaminant

More Related Videos

Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants
07:08

Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants

Published on: March 6, 2018

6.0K
Assessment of the Effects of Endocrine Disrupting Compounds on the Development of Vertebrate Neural Network Function Using Multi-electrode Arrays
08:28

Assessment of the Effects of Endocrine Disrupting Compounds on the Development of Vertebrate Neural Network Function Using Multi-electrode Arrays

Published on: April 26, 2018

5.9K

Related Experiment Videos

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation
17:28

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Published on: June 17, 2015

12.6K
Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants
07:08

Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants

Published on: March 6, 2018

6.0K
Assessment of the Effects of Endocrine Disrupting Compounds on the Development of Vertebrate Neural Network Function Using Multi-electrode Arrays
08:28

Assessment of the Effects of Endocrine Disrupting Compounds on the Development of Vertebrate Neural Network Function Using Multi-electrode Arrays

Published on: April 26, 2018

5.9K

Area of Science:

  • Environmental health
  • Developmental toxicology
  • Stem cell biology

Background:

  • Environmental chemicals are linked to adverse pregnancy outcomes.
  • The impact of chemical mixtures at environmentally relevant concentrations is understudied.
  • Nunavik pregnant women have measurable levels of various environmental chemicals.

Purpose of the Study:

  • To investigate the effects of the Nunavik Chemical Mixture (NCM) on human embryonic stem cells (hESCs).
  • To assess NCM's impact on critical embryonic development processes.
  • To understand potential interactions within the chemical mixture.

Main Methods:

  • hESCs were exposed to a mixture of 23 environmental chemicals (NCM) at varying concentrations.
  • Cell viability, apoptosis, cell cycle, cytoskeleton, and autophagy were analyzed.
  • Expression of lineage marker genes and proteins was measured.

    • Main Results:

    • NCM significantly decreased cell viability and adhesion in a dose-dependent manner.
    • Exposure to NCM induced apoptosis and disrupted the cell cycle.
    • Alterations in cytoskeleton, autophagy proteins, and lineage markers were observed, suggesting developmental toxicity.

    Conclusions:

    • The Nunavik Chemical Mixture (NCM) adversely affects human embryo development in vitro.
    • Observed effects differ from single-chemical exposures, highlighting mixture interactions.
    • Results underscore the need to consider combined chemical exposures for accurate health risk assessment.