Related Experiment Video

Updated: Jun 16, 2025

Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
04:41

Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue

Published on: July 28, 2023

2.2K

PRRX1 is a key regulator in the phenotypic transition between human normal dermal and keloid fibroblasts

Shuqian Dou1, Fengyu Zhang2, Yongjing He1

  • 1The Second Affiliated Hospital of Kunming Medical University, Kunming, China.

Journal of Dermatological Science
|June 14, 2025
PubMed

Insights

Human fetal skin heals without scars. Researchers identified paired related homeobox 1 (PRRX1) as a key factor in scarless wound healing, offering potential for new clinical treatments.

Area of Science:

  • Dermatology
  • Regenerative Medicine
  • Molecular Biology

Background:

  • Scarring post-skin trauma presents significant aesthetic and functional challenges.
  • Understanding scarless healing mechanisms is crucial for therapeutic advancements.

Purpose of the Study:

  • To investigate differences between fetal, postnatal, and keloid skin.
  • To identify key factors regulating scarless wound healing.

Main Methods:

  • Spatial transcriptomics (ST) and histological imaging were employed.
  • Comparative analysis of cellular and molecular characteristics across different skin types.

Main Results:

  • Histological and cellular distinctions were observed, including variations in extracellular matrix, appendages, stem cells, and immune cells.
  • Fibroblast heterogeneity was noted, with increased paired related homeobox 1 (PRRX1)-positive fibroblasts from fetal to keloid tissue.
  • PRRX1's role in fibroblast phenotypic transition and scarless healing was confirmed in a 3D keloid model.

Conclusions:

  • Fetal skin exhibits unique characteristics contributing to scarless healing.
  • PRRX1 is identified as a critical transcription factor for scarless wound healing.
  • Findings hold translational potential for developing clinical strategies to promote scarless healing.
Abstract

Keywords:
Human skinPRRX1Scarless wound healingSpatial transcriptomics

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