Antler base (Cervus nippon Temminck) peptides modulate the NLRP3 inflammatory pyroptosis and Nrf2/HO-1/NQO1 signaling pathways to ameliorate osteoarthritis: a structural and mechanistic study
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View abstract on PubMed
Summary
This summary is machine-generated.Cervus nippon Temminck antler base peptides (CNCPs) show potential in treating osteoarthritis by reducing inflammation and oxidative stress. These peptides regulate key signaling pathways, offering a novel therapeutic approach for osteoarthritis.
Area Of Science
- Biochemistry
- Pharmacology
- Molecular Biology
Background
- Antler base (Cervus nippon Temminck) is a traditional Chinese medicine used for osteoarthritis (OA).
- The molecular mechanisms and active compounds responsible for its therapeutic effects on OA remain largely unknown.
Purpose Of The Study
- To elucidate the mechanism of action and identify the active components of Cervus nippon Temminck antler base peptides (CNCPs) in ameliorating OA.
- To investigate the structural characteristics of bioactive CNCPs.
Main Methods
- Characterization of CNCPs using FTIR and HPLC.
- Evaluation of CNCPs' protective effects and mechanisms in a papain-induced OA rat model via histopathology, ELISA, Western blotting, qRT-PCR, and immunofluorescence.
- Isolation and purification of CNCPs using Sephadex G-15, bioactivity screening in an IL-1β-induced in vitro model, and identification of active components by LC-MS/MS.
Main Results
- CNCPs significantly reduced cartilage erosion, preserved cartilage integrity, modulated OA-related protein expression, and normalized serum levels of pro-inflammatory and oxidative stress markers in OA rats.
- CNCPs inhibited NLRP3 inflammasome-mediated pyroptosis and activated the Nrf2 pathway.
- The most active fraction, CNCPs-III (<1.5 kDa), was rich in hydrophobic amino acids like AGPAGA, VGPV, and LLGDV.
Conclusions
- CNCPs effectively mitigate OA-induced inflammation and oxidative stress.
- The therapeutic effects are mediated through the regulation of the NLRP3 inflammatory pyroptosis and Nrf2/HO-1/NQO1 signaling pathways.
- CNCPs demonstrate potential in delaying osteoarthritis progression.
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