Related Experiment Video

Updated: Sep 19, 2025

A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia
11:36

A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia

Published on: November 14, 2020

9.6K

G protein and MYD Growth Factor in neuroprotection after cerebral ischemia-reperfusion

Yisheng Cheng1, Xiaoxiao Cheng2, Han Zhao1

  • 1Department of Neurology, Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine, Wenzhou, Zhejiang, China.

Neurological Research
|June 15, 2025
PubMed
Abstract

Insights

Myeloid-Derived Growth Factor (MYDGF) protects the brain after stroke by activating key cell signaling pathways. Gαi1/3 is crucial for MYDGF

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biochemistry

Background:

  • Cerebral ischemia-reperfusion injury (CIRI) is a major cause of brain damage.
  • Understanding the molecular mechanisms underlying neuroprotection is critical for developing effective treatments.

Purpose of the Study:

  • To investigate the neuroprotective potential of Myeloid-Derived Growth Factor (MYDGF) in CIRI.
  • To elucidate the role of G protein-coupled signaling pathways in MYDGF-mediated neuroprotection.

Main Methods:

  • A mouse model of CIRI was established using middle cerebral artery occlusion (MCAO).
  • MYDGF and Gαi1/3 expression was modulated using shRNA and recombinant protein.
  • Gene and protein expression levels were analyzed using qRT-PCR and Western blot.
  • Cell-type specific knockdown in glial cells was performed using adenoviral shRNA.

Main Results:

  • MYDGF demonstrated significant neuroprotective effects in the CIRI model.
  • Activation of PI3K-AKT-mTOR and ERK-MAPK signaling pathways was observed.
  • Gαi1/3 was identified as a key mediator of MYDGF's neuroprotective actions.

Conclusions:

  • MYDGF confers neuroprotection against CIRI through the PI3K-AKT-mTOR and ERK-MAPK pathways.
  • Gαi1/3 plays a critical role in mediating the therapeutic effects of MYDGF.
Keywords:
G proteinMYDGFcerebral ischemia-reperfusion injuryneuroprotectionsignaling pathway

More Related Videos

The Application Of Permanent Middle Cerebral Artery Ligation in the Mouse08:27

The Application Of Permanent Middle Cerebral Artery Ligation in the Mouse

Published on: July 25, 2011

19.8K
Herbal Munziq Ameliorates Myocardial Ischemia-Reperfusion Injury by Inhibiting Inflammation09:53

Herbal Munziq Ameliorates Myocardial Ischemia-Reperfusion Injury by Inhibiting Inflammation

Published on: January 10, 2025

555

Related Concept Videos

Activation and Inactivation of G Proteins01:22

Activation and Inactivation of G Proteins

Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high...
7.7K
Neurogenesis and Regeneration of Nervous Tissue01:15

Neurogenesis and Regeneration of Nervous Tissue

In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...
1.1K