Development of a reference material for simultaneous quantitative determination of multiple veterinary drug residues

Excessive use of multiple veterinary drugs can lead to the accumulation of residues in food derived from animals, thereby posing potential risks to human health and the environment. Accurate quantification of residues from multiple veterinary drugs is essential for ensuring effective monitoring and regulatory compliance. In this study, a robust high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) method was developed for the simultaneous determination of six veterinary drug residues, including florfenicol (FF), thiamphenicol (TAP), difloxacin (DIF), sulfadimidine (SDM), trimethoprim (TMP), and fenvalerate (FEN). This method was subsequently applied to quantify the multiple veterinary reference material (MVRM). Using the MVRM with assigned values, the varying performance of pre-treatment methods for multiple veterinary drugs in meat samples was evaluated, which may result in differing quantitative outcomes. The application of MVRM is helpful for ensuring food safety and safeguarding human health.