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MdDUF506 Enhances Aluminium Tolerance by Interacting With MdCNR8 in Apple
Da-Ru Wang1, Ming-Hui Xu2, Xun Wang1
1Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, China.
Plant, Cell & Environment
|June 16, 2025
Summary
Apple rootstock
Area of Science:
- Plant Science
- Agronomy
- Molecular Biology
Background:
- Soil acidification from industrial and agricultural activities releases toxic aluminum ions (Al3+).
- Aluminum stress significantly inhibits plant growth, particularly roots and stems, due to phytotoxicity.
- Understanding plant responses to aluminum stress is crucial for crop resilience.
Purpose of the Study:
- Investigate aluminum stress tolerance in apple rootstocks 'YZ3' and 'YZ6'.
- Identify genes involved in aluminum stress response and tolerance mechanisms.
- Elucidate the function of a novel gene, MdDUF506, in mediating aluminum tolerance.
Main Methods:
- Comparative transcriptome analysis of apple rootstocks under aluminum stress.
- Gene expression analysis (overexpression) in apple and calli.
- Analysis of reactive oxygen species (ROS) scavenging.
- Gene interaction studies (MdDUF506 and MdCNR8).
Main Results:
- 'YZ3' rootstock demonstrated superior tolerance to aluminum stress compared to 'YZ6'.
- A differentially expressed gene, MdDUF506, was identified and its overexpression enhanced ROS scavenging.
- MdDUF506 positively regulates aluminum stress tolerance by modulating key stress-related genes (MdSTOP1, MdRSL1, MdRSL4, MdGL2, MdRAE1).
- MdDUF506 interacts with MdCNR8, contributing to enhanced aluminum stress tolerance.
Conclusions:
- MdDUF506 plays a critical role in enhancing aluminum stress tolerance in apple rootstocks.
- Overexpression of MdDUF506 mitigates oxidative damage and promotes plant growth under aluminum stress.
- These findings provide valuable candidate genes for breeding aluminum-tolerant apple varieties and offer insights into plant stress resistance mechanisms.
