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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Sep 19, 2025

Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry

Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry

Published on: September 28, 2016

Recommendations for Accurate Target Expression Evaluation by Quantitative Flow Cytometry.

Tamara Lekishvili¹, Maxime Moulard², Sarah Jetzer¹

¹Molecular Partners AG, Zürich, Switzerland.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology

|June 16, 2025

Summary

This summary is machine-generated.

This study enhances quantitative flow cytometry by identifying key factors affecting antibody binding capacity (ABC) measurements. Recommendations are provided for robust and reproducible antigen expression analysis.

Keywords:

antibody binding capacity quantitative flow cytometry receptor density standardization

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Related Experiment Videos

Last Updated: Sep 19, 2025

Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry

Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry

Published on: September 28, 2016

The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo

The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo

Published on: June 19, 2014

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

Published on: March 10, 2018

Area of Science:

Immunology
Cell Biology
Biotechnology

Background:

Flow cytometry quantifies cellular characteristics like antigen expression.
Antibody binding capacity (ABC) is crucial for estimating antigen density and ligand-binding sites.
Variability in reported ABC values necessitates assay standardization.

Purpose of the Study:

To address challenges in obtaining reproducible quantitative flow cytometry data.
To provide methodological recommendations for accurate antigen expression assessment.
To identify sources of variation in ABC calculations.

Main Methods:

Comprehensive investigation of factors influencing ABC values.
Evaluation of instruments (conventional and full-spectrum), antibodies, reagents, matrices, cell density, autofluorescence, and quantitative kits.
Implementation of a systematic and integrated approach.

Main Results:

Identified primary sources of variation in ABC calculations.
Demonstrated assay robustness through longitudinal studies.
Established a protocol supporting biomarker evaluation.

Conclusions:

Accurate antigen expression analysis requires standardized quantitative flow cytometry methods.
Methodological recommendations improve the reliability and reproducibility of ABC values.
The established protocol facilitates biomarker evaluation in various contexts.