Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

3.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K
Depressive Disorders: Etiology01:27

Depressive Disorders: Etiology

173
Depressive disorders result from a complex interplay of biological, psychological, and sociocultural factors, each contributing uniquely to the development and persistence of the condition. Understanding these factors provides critical insight into the multifaceted nature of depression.
Biological Factors in Depression
Biological predispositions significantly influence the risk of developing depressive disorders. Genetic studies highlight the role of variations in the serotonin transporter...
173
Depressive Disorders: MDD and Dysthymia01:27

Depressive Disorders: MDD and Dysthymia

225
Depressive disorders are a group of mental health conditions characterized by pervasive feelings of sadness, diminished pleasure in life, and a significant impact on daily functioning. These conditions are most prevalent in individuals during their 30s and affect women at twice the rate of men. Contrary to popular belief, younger individuals are generally more susceptible to these disorders than older adults. Two key types of depressive disorders include Major Depressive Disorder (MDD) and...
225
Depression: Overview01:18

Depression: Overview

371
Depression is a prevalent mental illness marked by persistent sadness and lack of interest in previously enjoyable activities. It can take several forms, including major depression, persistent depressive disorder, and bipolar I and II disorders. Symptoms range from emotional changes like chronic worry to physical changes like sleep disturbances and suicidal thoughts. From a neurobiological perspective, depression is believed to be triggered by abnormalities in the brain's prefrontal cortex,...
371
Long-term Depression01:05

Long-term Depression

31.5K
Long-term depression, or LTD, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTD is the process of synaptic weakening that occurs over time between pre and postsynaptic neuronal connections. The synaptic weakening of LTD works in opposition to synaptic strengthening by long-term potentiation (LTP) and together are the main mechanisms that underlie learning and memory.
31.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Isolation of Small Extracellular Vesicles from Murine Skeletal Muscle and Bone Marrow by Size-Exclusion Chromatography.

Journal of visualized experiments : JoVE·2026
Same author

Multi-omics characterization of astrocyte subtypes reveals spatially coordinated astrocyte downregulation in depression.

bioRxiv : the preprint server for biology·2026
Same author

The functional relevance of a short assessment of formal thought disorder in psychosis.

The British journal of psychiatry : the journal of mental science·2026
Same author

Correction: A genome-wide investigation of depression among individuals with and without irritability.

Molecular psychiatry·2026
Same author

Astrocyte fatty acid metabolism as a driver of risk for major depressive disorder.

Nature communications·2026
Same author

Characterization of the chromosome 7 locus associated with suicidal behavior.

Molecular psychiatry·2026

Related Experiment Video

Updated: Sep 19, 2025

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
08:22

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

8.2K

Peripheral MicroRNA Signatures in Adolescent Depression.

Alice Morgunova1,2,3, Nicholas O'Toole2,4, Fatme Abboud2

  • 1Integrated Program in Neuroscience, McGill University, Montreal, Quebec, Canada.

Biological Psychiatry Global Open Science
|June 16, 2025
PubMed
Summary

Researchers identified specific microRNAs (miRNAs) in adolescents with depression. Some miRNAs correlated with depression severity and hippocampal volume, suggesting potential biomarkers for lifelong mental health risk.

Keywords:
AdolescenceDepressionDevelopmentDried blood spotsMental healthMicroRNA

More Related Videos

The Unpredictable Chronic Mild Stress Protocol for Inducing Anhedonia in Mice
07:13

The Unpredictable Chronic Mild Stress Protocol for Inducing Anhedonia in Mice

Published on: October 24, 2018

17.7K
Author Spotlight: Understanding Adolescent Social Adversity Effects on Neurodevelopment in Mice
07:15

Author Spotlight: Understanding Adolescent Social Adversity Effects on Neurodevelopment in Mice

Published on: March 15, 2024

1.8K

Related Experiment Videos

Last Updated: Sep 19, 2025

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
08:22

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

8.2K
The Unpredictable Chronic Mild Stress Protocol for Inducing Anhedonia in Mice
07:13

The Unpredictable Chronic Mild Stress Protocol for Inducing Anhedonia in Mice

Published on: October 24, 2018

17.7K
Author Spotlight: Understanding Adolescent Social Adversity Effects on Neurodevelopment in Mice
07:15

Author Spotlight: Understanding Adolescent Social Adversity Effects on Neurodevelopment in Mice

Published on: March 15, 2024

1.8K

Area of Science:

  • Neuroscience
  • Genetics
  • Psychiatry

Background:

  • Adolescent depression has long-term negative consequences and poor treatment outcomes.
  • Early identification of epigenetic markers is crucial for effective interventions.
  • The role of microRNAs (miRNAs) in adolescent depression is not well understood.

Purpose of the Study:

  • To investigate miRNA profiles in adolescents with clinical depression.
  • To identify potential peripheral miRNA biomarkers for adolescent depression.

Main Methods:

  • Small RNA sequencing was performed on dried blood spot samples from adolescents with and without depression.
  • Analysis focused on identifying differentially expressed miRNAs.

Main Results:

  • Nine differentially expressed miRNAs were found, all upregulated in depressed adolescents.
  • Specific miRNAs (miR-3613-5p, mir-30c-2, miR-942-5p) correlated with depression severity.
  • miR-32-5p expression was inversely associated with hippocampal volume.

Conclusions:

  • Peripheral miRNAs may serve as biomarkers for adolescent depression.
  • These findings highlight miRNAs involved in neurodevelopmental pathways linked to psychopathology.
  • This research provides a foundation for developing novel diagnostic and therapeutic strategies.