Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Computational study of H<sub>2</sub> generation from BH<sub>3</sub> and BH<sub>3</sub> <sup>-</sup> with HO˙ radical.

RSC advances·2026
Same author

Computational Drug Repurposing Predicts FDA-Approved Drugs as Potential Inhibitors of Chikungunya Virus nsP2 Protease.

The journal of physical chemistry. B·2026
Same author

Inhibitory effect of thiamine salts on steel corrosion in an acidic environment: an experimental and theoretical approach.

RSC advances·2026
Same author

Electrochemical determination of ciprofloxacin using a MIL-101/reduced graphene oxide-modified electrode.

Beilstein journal of nanotechnology·2026
Same author

Dynamics of Aβ42 Tetramer by REST2-CHARMM36m Simulations.

The journal of physical chemistry. B·2026
Same author

The environmental degradation of naphthalimide, rhodamine and BODIPY fluorophores by hydroxyl radicals: a theoretical insight.

RSC advances·2026

Related Experiment Video

Updated: Jun 16, 2026

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

13.0K

A mercury complex-based fluorescent sensor for biological thiols.

Nguyen Khoa Hien1, Trinh Thi Giao Chau1, Nguyen Dinh Luyen2

  • 1Mientrung Institute for Scientific Research, Vietnam National Museum of Nature, Vietnam Academy of Science and Technology Hue 49000 Vietnam.

RSC Advances
|June 16, 2025
PubMed
Summary

A new fluorescent sensor, Hg(DST)2, selectively detects biological thiols like glutathione (GSH), cysteine (Cys), and homocysteine (Hcy) in water. This sensor offers a sensitive and selective method for quantifying these vital biomolecules.

More Related Videos

A Study of the Complexation of MercuryII with Dicysteinyl Tetrapeptides by Electrospray Ionization Mass Spectrometry
12:59

A Study of the Complexation of MercuryII with Dicysteinyl Tetrapeptides by Electrospray Ionization Mass Spectrometry

Published on: January 8, 2016

8.1K
An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium
09:33

An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium

Published on: December 17, 2018

10.3K

Related Experiment Videos

Last Updated: Jun 16, 2026

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

13.0K
A Study of the Complexation of MercuryII with Dicysteinyl Tetrapeptides by Electrospray Ionization Mass Spectrometry
12:59

A Study of the Complexation of MercuryII with Dicysteinyl Tetrapeptides by Electrospray Ionization Mass Spectrometry

Published on: January 8, 2016

8.1K
An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium
09:33

An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium

Published on: December 17, 2018

10.3K

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Materials Science

Background:

  • Biological thiols, including glutathione (GSH), cysteine (Cys), and homocysteine (Hcy), play critical roles in cellular processes.
  • Accurate quantification of these thiols is essential for understanding various physiological and pathological conditions.
  • Development of selective and sensitive detection methods for thiols in aqueous environments remains a challenge.

Purpose of the Study:

  • To develop a novel fluorescent sensor, Hg(DST)2, for the selective detection of biological thiols.
  • To investigate the sensing mechanism and evaluate the sensor's performance in aqueous solutions.
  • To demonstrate the sensor's applicability in biological and environmental sample analysis.

Main Methods:

  • Synthesis and characterization of the Hg(DST)2 fluorescent sensor.
  • Fluorescence spectroscopy for detecting thiols.
  • Density Functional Theory (DFT) calculations to elucidate the sensing mechanism.
  • Interference studies with common biomolecules and metal ions.
  • Method validation using fluorescence titration and limit of detection analysis.

Main Results:

  • Hg(DST)2 exhibited selective fluorescence quenching upon Hg2+ coordination, with reversible recovery in the presence of thiols.
  • The sensor demonstrated high sensitivity and selectivity for GSH, Cys, and Hcy in aqueous solutions at pH 7.2.
  • Linear correlations were observed between fluorescence intensity and thiol concentrations, with low limits of detection (LODs) ranging from 0.26 to 0.47 μM.
  • Interference from other species was minimal, and specific metal ion interferences were mitigated using 1,10-phenanthroline (PHEN).

Conclusions:

  • Hg(DST)2 is a highly sensitive and selective fluorescent sensor for biological thiols in aqueous media.
  • The sensor operates via an OFF-ON fluorescence mechanism attributed to Hg-thiol complex formation.
  • Hg(DST)2 shows significant potential for the quantification of thiols in complex biological and environmental samples.