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Induction and Micro-CT Imaging of Cerebral Cavernous Malformations in Mouse Model
Published on: September 4, 2017
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Single-cell transcriptome profiling reveals dynamic cell populations and immune infiltration in cerebral cavernous
Zhiguang Han1, Chengxu Lei2, Zhenyu Zhou3
1Department of Neurosurgery, The First Hospital of Qinhuangdao, Hebei, China.
Frontiers in Immunology
|June 16, 2025
Summary
Cerebral cavernous malformation (CCM) involves increased immune cells like monocytes, neutrophils, and NK cells in lesions. This study reveals altered gene expression and enhanced cell communication, offering potential therapeutic targets for CCM.
Area of Science:
- Neuroscience
- Immunology
- Genomics
Background:
- Cerebral cavernous malformation (CCM) pathogenesis and cellular landscape are not fully understood.
- Identifying cellular subpopulations and signaling pathways in CCM lesions is crucial for understanding disease mechanisms.
Purpose of the Study:
- To comprehensively map cellular subpopulations and signaling pathway alterations in sporadic CCM patient tissues.
- To elucidate the molecular and cellular basis of CCM pathogenesis.
Main Methods:
- Single-cell RNA sequencing and multiplex fluorescent immunohistochemistry on CCM lesional tissues.
- Differential gene expression analysis, pathway enrichment, and cell-cell communication analysis.
Main Results:
- Identified 8 major cell types in CCM lesions, with increased monocytes, neutrophils, and NK cells.
- Discovered 28 differentially expressed genes and altered pathways, including NK cell-mediated cytotoxicity.
- Observed enhanced intercellular communication in CCM lesion tissues.
Conclusions:
- Single-cell transcriptomics reveals increased immune cell infiltration and dysregulated pathways in CCM.
- Enhanced cell communication via VEGF and ADGRE5 pathways suggests potential therapeutic targets for CCM.
