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Updated: Sep 19, 2025
Hybrid µCT-FMT imaging and image analysis
Published on: June 4, 2015
LEVERAGING CONTRAST AGENT KINETICS FOR ROBUST REFLECTANCE MODE FLUORESCENCE TOMOGRAPHY
Mariella Kast1, Mykhaylo Zayats2, Shayan Shafiee3
1MCSS, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland.
Abstract:
Fluorescence Image Guided Surgery utilizes continuous wave epi-fluorescence measurements on the tissue surface to locate targets such as tumors or lymph nodes, but precise 3D localization of deep targets remains intractable due to the illposedness of the associated inverse problem. We propose a Fluorescence Diffuse Optical Tomography scheme which leverages the different contrast agent kinetics in malignant vs normal tissue and reconstructs the 3D tumor location from a time series of epi-fluorescence measurements. We conduct sequential synthetic experiments, which mimic the differential uptake and release profile of fluorescent dye ICG in tumors vs normal tissue and demonstrate for the first time that the proposed method can robustly recover targets up to 1cm deep and in the presence of realistic tumor-to-background ratios.
Insights
This study introduces a new Fluorescence Diffuse Optical Tomography method for 3D tumor localization in surgery. The technique accurately identifies deep targets using contrast agent kinetics, improving surgical precision.
Area of Science:
- Medical Imaging
- Optical Tomography
- Surgical Navigation
Background:
- Fluorescence Image Guided Surgery (FIGS) uses surface measurements for target localization.
- Precise 3D localization of deep surgical targets remains challenging due to inverse problem complexities.
Purpose of the Study:
- To develop a Fluorescence Diffuse Optical Tomography (FDOT) scheme for accurate 3D localization of deep tumors.
- To leverage differential contrast agent kinetics between malignant and normal tissues.
Main Methods:
- Proposed an FDOT scheme reconstructing 3D tumor location from time-series epi-fluorescence measurements.
- Conducted sequential synthetic experiments mimicking Indocyanine Green (ICG) dye kinetics in tumors versus normal tissue.
Main Results:
- Successfully demonstrated robust 3D localization of targets up to 1cm deep.
- Achieved accurate reconstruction in the presence of realistic tumor-to-background ratios.
Conclusions:
- The proposed FDOT method offers a viable solution for precise 3D localization of deep surgical targets.
- This approach enhances Fluorescence Image Guided Surgery by overcoming limitations of surface measurements.
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