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Total Internal Reflection Fluorescence Microscopy01:05

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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LEVERAGING CONTRAST AGENT KINETICS FOR ROBUST REFLECTANCE MODE FLUORESCENCE TOMOGRAPHY.

Mariella Kast1, Mykhaylo Zayats2, Shayan Shafiee3

  • 1MCSS, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland.

Proceedings. IEEE International Symposium on Biomedical Imaging
|June 16, 2025
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Summary
This summary is machine-generated.

This study introduces a new Fluorescence Diffuse Optical Tomography method for 3D tumor localization in surgery. The technique accurately identifies deep targets using contrast agent kinetics, improving surgical precision.

Keywords:
ICG pharmacokineticsNIR Optical tomographyfluorescence imaging

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Area of Science:

  • Medical Imaging
  • Optical Tomography
  • Surgical Navigation

Background:

  • Fluorescence Image Guided Surgery (FIGS) uses surface measurements for target localization.
  • Precise 3D localization of deep surgical targets remains challenging due to inverse problem complexities.

Purpose of the Study:

  • To develop a Fluorescence Diffuse Optical Tomography (FDOT) scheme for accurate 3D localization of deep tumors.
  • To leverage differential contrast agent kinetics between malignant and normal tissues.

Main Methods:

  • Proposed an FDOT scheme reconstructing 3D tumor location from time-series epi-fluorescence measurements.
  • Conducted sequential synthetic experiments mimicking Indocyanine Green (ICG) dye kinetics in tumors versus normal tissue.

Main Results:

  • Successfully demonstrated robust 3D localization of targets up to 1cm deep.
  • Achieved accurate reconstruction in the presence of realistic tumor-to-background ratios.

Conclusions:

  • The proposed FDOT method offers a viable solution for precise 3D localization of deep surgical targets.
  • This approach enhances Fluorescence Image Guided Surgery by overcoming limitations of surface measurements.