Related Experiment Video
Updated: Jun 9, 2026
12:00
Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro
Published on: July 5, 2017
19.3K
Enhancing Phenolic Compound Recovery from Grape Pomace Residue: Synergistic Approach of Ultrasound- and
Natalia Stanek-Wandzel1, Magdalena Zarębska1, Tomasz Wasilewski1,2
1Institute of Heavy Organic Synthesis "Blachownia", Łukasiewicz Research Network, Energetykow 9, 47-225 Kedzierzyn-Kozle, Poland.
ACS Omega
|June 16, 2025
Summary
A novel ultrasound- and enzyme-assisted extraction (UEAE) method efficiently isolates phenolic compounds from grape pomace. This combined approach significantly enhances the recovery of valuable bioactive compounds compared to traditional methods.
Area of Science:
- Food Chemistry
- Extraction Technology
- Biotechnology
Background:
- Grape pomace is a rich source of bioactive phenolic compounds.
- Conventional extraction methods often have limitations in efficiency and compound recovery.
- Developing advanced extraction techniques is crucial for valorizing grape pomace.
Purpose of the Study:
- To develop and optimize a novel ultrasound- and enzyme-assisted extraction (UEAE) method for phenolic compounds from red and white grape pomace.
- To evaluate the impact of key process parameters on extraction efficiency and phenolic profile.
- To compare UEAE with solid-liquid extraction (SLE), ultrasound-assisted extraction (UAE), and enzyme-assisted extraction (EAE).
Main Methods:
- Optimization of enzyme mixture composition, pH, hydrolysis time, and sonication duration.
- Application of ultrasound post-enzymatic hydrolysis.
- Comparative analysis with SLE, UAE, and EAE methods.
Main Results:
- UEAE significantly enhanced the release of both free and cell-wall-bound phenolic compounds.
- The method effectively extracted phenolic acids (gallic, caffeic, ferulic, p-coumaric), flavonoids (quercetin, kaempferol, resveratrol), and anthocyanins (malvidin-3-glucoside).
- Highest total phenolic content and antioxidant activity were achieved with ultrasound following 60 min of enzymatic hydrolysis.
Conclusions:
- The optimized UEAE method is highly effective for maximizing the recovery of diverse bioactive compounds from grape pomace.
- Ultrasound's mechanical disruption aids in releasing phenolics bound within the plant cell structure.
- UEAE presents an efficient and versatile approach for grape pomace valorization.
