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Updated: Sep 19, 2025
10:47
Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI
Published on: March 24, 2016
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Structure Elucidation for MALDI Mass Spectrometry Imaging Using Infrared Ion Spectroscopy
Jelle L Schuurman1, Lara van Tetering1, Kas J Houthuijs1
1HFML-FELIX, Radboud University, 6525 ED Nijmegen, The Netherlands.
Analytical Chemistry
|June 16, 2025
Summary
Matrix-assisted laser desorption/ionization with infrared ion spectroscopy (MALDI-IRIS) directly analyzes small molecules in biological tissues. This advance aids metabolite identification in complex samples like brain tissue, improving disease research.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Spectroscopy
Background:
- Infrared ion spectroscopy (IRIS) is a tandem mass spectrometry (MS) technique for ion structural analysis.
- Previous IRIS applications focused on solution-based biological samples using electrospray ionization (ESI) coupled with liquid chromatography-mass spectrometry (LC-MS).
- Direct analysis of small molecules from complex biological tissues using IRIS has been limited.
Purpose of the Study:
- To combine matrix-assisted laser desorption/ionization (MALDI) with IRIS for direct analysis of small molecules from biological tissues.
- To apply this novel MALDI-IRIS platform to analyze brain tissue from mouse models of l-lysine catabolism disorders.
- To demonstrate the potential for structural characterization of metabolites in complex biological tissues using MALDI-IRIS.
Main Methods:
- Developed and applied a matrix-assisted laser desorption/ionization with infrared ion spectroscopy (MALDI-IRIS) platform.
- Utilized Fourier-transform ion cyclotron resonance mass spectrometry for analysis.
- Performed MALDI mass spectrometry imaging on brain tissue from pyridoxine-dependent epilepsy (ALDH7A1) and glutaric aciduria type 1 (GCDH) mouse models.
Main Results:
- Successfully combined MALDI with IRIS for direct structural analysis of small molecules in biological tissues.
- Analyzed brain tissue from specific knockout mouse models, demonstrating the technique's applicability to disease research.
- Generated structurally diagnostic vibrational spectra for mass-selected ions directly from tissue samples.
Conclusions:
- The MALDI-IRIS platform enables direct structural characterization of metabolites within complex biological tissues.
- This technique significantly advances the capabilities of MALDI mass spectrometry imaging.
- Opens new avenues for metabolite structure elucidation and understanding of metabolic disorders.
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