Advancing Biomarker Detection with Peptide-Integrated Fluorescent Probes.

Area of Science:

• Biomedical Engineering
• Molecular Biology
• Analytical Chemistry

Background:

• Over-expressed biomarkers are indicators of various diseases.
• Optical probes enable timely disease detection and prediction.
• Probe biocompatibility is a critical challenge in optical sensing.

Purpose of the Study:

• To review recent developments in peptide-based fluorescent probes.
• To highlight the use of peptides as adaptable molecular templates for biosensors.
• To focus on the detection of various disease-related biomarkers.

Main Methods:

• Exploration of peptide-based sensors for nucleic acid detection (miRNA, DNA, RNA).
• Investigation of peptide-based enzyme-responsive biosensors and conjugates.
• Review of peptide templates for fabricating fluorescent probes.

Main Results:

• Peptides offer excellent biocompatibility and target selectivity.
• Peptidyl templates facilitate easy synthesis and flexible structures.
• Peptide-based probes show promise for detecting enzymes like cathepsin-B.

Conclusions:

• Peptide-based fluorescent probes are a promising strategy for biomarker detection.
• The biocompatibility and adaptability of peptides enhance biosensor performance.
• Recent developments show significant progress in peptide-based optical sensing for diseases.