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Updated: Sep 19, 2025

Using Optical Tweezers for the Generation of Hybrid Spheroids
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Using Optical Tweezers for the Generation of Hybrid Spheroids

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Using Optical Tweezers for the Generation of Hybrid Spheroids.

Kamila Duś-Szachniewicz1, Katarzyna Gdesz-Birula2, Sławomir Drobczyński3

  • 1Department of Clinical and Experimental Pathology, Institute of General and Experimental Pathology, Wrocław Medical University; kamila.dus-szachniewicz@umw.edu.pl.

Journal of Visualized Experiments : Jove
|June 16, 2025
PubMed
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Optical tweezers (OT) offer a novel, non-invasive method for creating 3D lymphoma-stromal cell spheroids. This technique precisely controls cell attachment and adhesion, advancing cancer research and drug testing.

Area of Science:

  • Biomedical Engineering
  • Cancer Research
  • Cell Biology

Background:

  • Two-dimensional (2D) cell cultures are standard in preclinical cancer research but lack in vivo accuracy.
  • Three-dimensional (3D) in vitro models offer more relevant architectural morphology, microenvironment, and drug responses.
  • Optical trapping (OT) uses lasers to manipulate microscale structures, with underutilized potential in cancer research.

Purpose of the Study:

  • To introduce a non-invasive protocol for forming hybrid lymphoma-stromal cell spheroids using optical tweezers (OT).
  • To demonstrate OT's capability in controlling cell attachment and adhesion timing for tissue engineering applications.
  • To highlight OT's utility in studying early spheroid formation and drug-induced adhesion changes.

Main Methods:

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  • Utilized optical tweezers (OT) for non-invasive manipulation of lymphoma-stromal cells.
  • Developed a protocol for de novo construction of hybrid spheroids.
  • Controlled the number of attached cells and adhesion formation time.

Main Results:

  • Successfully formed hybrid lymphoma-stromal cell spheroids with controlled cell numbers and adhesion.
  • Enabled study of early spheroid formation stages, surpassing standard bulk techniques.
  • Established a model for investigating minimal adhesion changes induced by anti-cancer drugs.

Conclusions:

  • Optical tweezers provide a versatile, non-invasive method for 3D spheroid formation in cancer research.
  • The protocol offers precise control over spheroid construction, valuable for tissue engineering.
  • This model facilitates the study of drug effects on cell adhesion, applicable to various cell types.