Related Experiment Video
Updated: Sep 19, 2025
Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
Published on: February 14, 2018
Molecular Networking-Driven Discovery of Antifungal Azaphilone Dimers from the Marine-Derived Fungus Humicola sp.
Min Chen1, Zhong-Hui Li2, Xia-Hao Zhu1
1Key Laboratory of Cultivated Land Quality Monitoring and Evaluation, Ministry of Agriculture and Rural Affairs, College of Environmental Science and Engineering, Yangzhou University, Yangzhou 225000, People's Republic of China.
Abstract:
A novel azaphilone dimer, humilone A (1), along with its related monomer, humilone B (2), was isolated from cultures of the marine-derived fungus Humicola sp. HK1-8 using a molecular networking-guided discovery approach. Further investigation of the molecular family of azaphilone dimers led to the putative identification of four analogues, humilones C-F (3-6), based on systematic analysis of their characteristic MS/MS fragmentation patterns. Detailed fragmentation studies of the dimers revealed that the predominant cleavage fragments originated from C-C bond scission at the dimeric methylene bridge. Compound 1 displayed antifungal activity against Rhizoctonia solani.
