Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Formation of Intermediate Filaments

Formation of Intermediate Filaments

Intermediate filaments are cytoskeletal proteins with higher tensile strength and flexibility than microfilaments and microtubules. Unlike the other two cytoskeletal proteins, intermediate filament formation lacks the enzymatic activity to hydrolyze nucleotides like ATP and GTP to generate energy for polymerization. Therefore, the formation of intermediate filaments is multistep self-assembly. The involvement of any accessory proteins in intermediate filament formation has not yet been reported.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Nitric oxide drives proteomic diversity through alternative splicing.

Molecular cell·2026

Same author

A small-molecule stabilizer of the calpastatin-calpain-2 complex restores mitochondrial function and mitigates neurodegeneration.

Science advances·2026

Same author

Optimization of 3D printing parameters for PLGA/HA scaffolds using the Taguchi method.

Journal of the mechanical behavior of biomedical materials·2026

Same author

Computed microtomographic imaging of revascularization during healing of Achilles tendon injury.

Journal of anatomy·2025

Same author

Pirfenidone Delivery by Blow-Molded PCL Nanofiber Mat to Reduce Collagen Synthesis by Fibroblasts.

Journal of biomedical materials research. Part A·2025

Same author

Sequential irradiation does not improve fatigue crack propagation resistance of human cortical bone at 15 kGy.

Journal of the mechanical behavior of biomedical materials·2024

Same journal

ROS-Responsive Quercetin Nanoparticles Improve the Prognosis of Traumatic Brain Injury by Inhibiting Aberrant Nrf2-Keap1 Signaling Pathway Activation.

Journal of biomedical materials research. Part A·2026

Same journal

Cellular Insights Into Proangiogenic Activation in Fibroblast and Endothelial Cells by Dual Drug-Loaded Emulsion Electrospun Nanofibers for Enhanced Tissue Regeneration.

Journal of biomedical materials research. Part A·2026

Same journal

Biomimetic Collagen Scaffolds Natural Cross-Linking Strategies via Transglutaminase and Methylglyoxal for Skin Repair.

Journal of biomedical materials research. Part A·2026

Same journal

Granular Hydrogel Composites for Noninvasive Optical Biosensing.

Journal of biomedical materials research. Part A·2026

Same journal

Performance Evaluation of Highly Uniform Astragalus Polysaccharide/Silk Fibroin Microspheres Fabricated via Microfluidics as Biodegradable Fillers: Collagen Regeneration and Tissue Reaction.

Journal of biomedical materials research. Part A·2026

Same journal

Combining Supramolecular and Covalent Chemistry to Form Reinforced Fibrillar Network Hydrogels From Fibrinogen Derivatives.

Journal of biomedical materials research. Part A·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 30, 2026

Postproduction Processing of Electrospun Fibres for Tissue Engineering

Postproduction Processing of Electrospun Fibres for Tissue Engineering

Published on: August 9, 2012

Tendon-Tissue Derived Monofilaments by Electrochemical Compaction: Production and Characterization.

Phillip McClellan¹, Joohee Choi¹, Mikhail Nasrallah²

¹Department of Mechanical and Aerospace Engineering, Case Western Reserve University, Cleveland, Ohio, USA.

Journal of Biomedical Materials Research. Part A

|June 16, 2025

Summary

This summary is machine-generated.

Researchers developed a novel method to process decellularized tendon powder into aligned fibers, creating a promising material for tendon tissue repair. This new tendon-derived thread (TDT) retains key proteins and supports cell growth.

Keywords:

decellularization electrochemical compaction tendon repair

More Related Videos

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization

Published on: April 19, 2015

Polytetrafluoroethylene PTFE as a Suture Material in Tendon Surgery

Polytetrafluoroethylene PTFE as a Suture Material in Tendon Surgery

Published on: October 6, 2022

Related Experiment Videos

Last Updated: Jun 30, 2026

Postproduction Processing of Electrospun Fibres for Tissue Engineering

Postproduction Processing of Electrospun Fibres for Tissue Engineering

Published on: August 9, 2012

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization

Published on: April 19, 2015

Polytetrafluoroethylene PTFE as a Suture Material in Tendon Surgery

Polytetrafluoroethylene PTFE as a Suture Material in Tendon Surgery

Published on: October 6, 2022

Area of Science:

Biomaterials Science
Orthopedic Surgery
Tissue Engineering

Background:

Tendon repair is challenging due to limitations in current graft materials.
Existing tendon grafts lack porosity and proper processing, hindering their full potential.

Purpose of the Study:

To develop a novel method for processing decellularized tendon into a scaffold for tissue repair.
To evaluate the composition and cellular compatibility of the new scaffold material.

Main Methods:

Native tendons were cryomilled and decellularized.
Solubilized collagen was electrochemically compacted to form aligned fibers (tendon-derived thread - TDT).
Proteomic analysis and cell culture assays were performed.

Main Results:

Decellularized tendon powder showed significantly reduced DNA content.
Proteomic analysis confirmed the presence of essential tendon proteins in TDT.
TDT retained proteoglycans, unlike pure collagen threads.
Mesenchymal stem cells showed similar gene expression on TDT and collagen threads.

Conclusions:

A novel method successfully produced tendon-derived threads (TDT) from decellularized tendon.
TDT preserves key extracellular matrix proteins and proteoglycans.
The TDT scaffold supports mesenchymal stem cell gene expression, indicating potential for tendon regeneration.