Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Two structural forms of histone octamer].

S N Khrapunov, A F Protas, A I Dragan

    Biofizika
    |September 1, 1985
    PubMed
    Summary

    Calf thymus histone octamers exhibit distinct structural states, transitioning from a loose to a compact form with increasing salt concentration. This structural change impacts tyrosyl accessibility and histone H3 stability.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Thermodynamic basis of the α-helix and DNA duplex.

    European biophysics journal : EBJ·2021
    Same author

    SYBR Green I: fluorescence properties and interaction with DNA.

    Journal of fluorescence·2012
    Same author

    Excitation volumetric effects (EVE) in metal-enhanced fluorescence.

    Physical chemistry chemical physics : PCCP·2011
    Same author

    Characterization of PicoGreen interaction with dsDNA and the origin of its fluorescence enhancement upon binding.

    Biophysical journal·2010
    Same author

    Metal-enhanced PicoGreen fluorescence: application to fast and ultra-sensitive pg/ml DNA quantitation.

    Journal of immunological methods·2010
    Same author

    Metal-Enhanced Fluorescence (MEF): Physical Characterization of Siver-Island Films and Exploring Sample Geometries.

    Chemical physics letters·2010

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Chromatin Structure

    Context:

    • Histone octamers are fundamental units of chromatin.
    • Understanding histone octamer structural dynamics is crucial for gene regulation.
    • Calf thymus histone octamers serve as a model system.

    Purpose:

    • To investigate the structural states of calf thymus histone octamers.
    • To characterize the properties of loose and compact histone octamer states.
    • To elucidate the role of salt concentration in histone octamer conformation.

    Summary:

    • Calf thymus histone octamers exist in two conformations: a loose state at 2M NaCl and a compact state at 4M NaCl.
    • The compact state shows reduced tyrosyl accessibility to quenching ions, longer tyrosyl relaxation times, enhanced histone H3 stability against trypsin, and no H3 SH-group interaction with parachloromercuribenzoate.

    Impact:

    • Provides insights into histone octamer structural plasticity.
    • Suggests a mechanism for modulating chromatin accessibility and function.
    • Contributes to understanding the biophysical basis of chromatin condensation.

    Related Experiment Videos