Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Benchtop Fabrication and Integration of Laser-Induced Graphene Strain Gauges and Stimulation Electrodes in Muscle on a Chip Devices.

Advanced functional materials·2026

Same author

Air pollution is linked to divergent cortical thickness patterns in brain regions vulnerable to Alzheimer's disease.

Neurotoxicology·2026

Same author

Association between late-life air pollution exposure and medial temporal lobe atrophy in older women.

Neurotoxicology·2026

Same author

Clinical and molecular characterization of <i>AXL</i> in colorectal cancer, CALGB (Alliance)/SWOG 80405 and real-world data.

Journal for immunotherapy of cancer·2025

Same author

DNA methylation: a potential mediator between air pollution exposures and asthma control.

Clinical epigenetics·2025

Same author

GABAergic signaling contributes to tumor cell invasion and poor overall survival in colorectal cancer.

Oncogene·2025

Same journal

Polarization-State-Dependent Charge Screening in Metal-Ferroelectric-Metal Memcapacitors Enabled by an IGZO Oxygen Reservoir Layer.

ACS applied materials & interfaces·2026

Same journal

Enabling Closed-Loop Recycling of Carbon Fiber-Reinforced Composites: A Dynamic Network Strategy Based on Cardanol-Derived Amines and Lignin-Derived Carbonates.

ACS applied materials & interfaces·2026

Same journal

Unconventional Phase Shift in Spin Hall Magnetoresistance of Antiferromagnetic Insulators.

ACS applied materials & interfaces·2026

Same journal

The Evolving Landscape of Terahertz Biosensing: From Sensitivity to Precision.

ACS applied materials & interfaces·2026

Same journal

π-π Stacking Enhanced Generation of Reactive Species in Donor-Acceptor Heterojunctions for High-Efficiency Photocatalytic Degradation of Endocrine-Disrupting Compounds under Solar Light.

ACS applied materials & interfaces·2026

Same journal

Interfacial Engineering of Frustrated Lewis Pairs for Promoting Cellulose-to-Sorbitol Cascade Conversion.

ACS applied materials & interfaces·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 19, 2025

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

A Permeabilization Workflow To Enable Specific Multiplexed Profiling Using SERS Nanoparticles.

Pegah Bagheri^1,2, Olga E Eremina^1,2, Nicholas Dorgan^1,2

¹Alfred E. Mann Department of Biomedical Engineering, University of Southern California, 36- McClintock Ave, Los Angeles, California 90089, United States.

ACS Applied Materials & Interfaces

|June 17, 2025

Summary

This summary is machine-generated.

Researchers developed a new method using surface-enhanced Raman scattering nanoparticles (SERS NPs) for improved intracellular breast cancer biomarker detection. This strategy enhances cell permeability, enabling accurate targeting of both extracellular and intracellular markers like HER2, ER, and PR.

Keywords:

Raman spectroscopy SERS nanoparticles breast cancer cell imaging cell permeabilization multiplexed imaging

More Related Videos

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Related Experiment Videos

Last Updated: Sep 19, 2025

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Area of Science:

Biomedical Engineering
Nanotechnology
Cancer Diagnostics

Background:

Surface-enhanced Raman scattering nanoparticles (SERS NPs) show promise for cellular targeting and biomarker detection.
Current limitations include poor membrane permeability and endosomal trapping, hindering intracellular applications.

Purpose of the Study:

To develop and optimize a SERS NP-based strategy for robust intracellular targeting of breast cancer biomarkers.
To demonstrate multiplexed detection of both extracellular (HER2) and intracellular (ER, PR) targets.

Main Methods:

Conjugation of SERS NPs with antibodies against HER2, ER, and PR.
Evaluation of permeabilization agents (Tween 20, Triton X-100, methanol) for intracellular delivery.
Assessment of SERS NP binding specificity and cell viability using flow cytometry, fluorescence, and Raman imaging.

Main Results:

Triton X-100 was identified as the optimal permeabilization agent, allowing SERS NP entry while maintaining cell viability.
High specificity (>100-fold) was observed for HER2-overexpressing cells.
Successful intracellular targeting and detection of ER and PR were achieved, alongside multiplexed detection of HER2, ER, and PR.

Conclusions:

The optimized SERS NP strategy enables efficient intracellular biomarker detection in breast cancer cells.
This platform offers potential for enhanced, multiplexed diagnostics, including applications in liquid biopsies.
The findings advance the use of SERS NPs for improved breast cancer diagnosis and personalized medicine.