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Updated: Sep 8, 2025

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
Published on: July 12, 2013
Large Stokes shift by increasing intramolecular charge transfer for high signal-to-background ratio bioimaging
Shiyu Wu1,2, Zhicheng Ban1,2, Hui Tang1,2
1Department of Chemistry, School of Science, Xihua University, Chengdu, 610039, China. yahuizhang@mail.xhu.edu.cn.
Abstract:
Novel bicyclic 2-pyridone probes with strong electron donors (from Br in cDHIP-N-Br to dimethoxytriphenylamine in cDHIP-N-MTPA) exhibit redshifted emission (535-759 nm) and large Stokes shifts (≤320 nm) due to ICT effects, enabling high-contrast bioimaging in cells, tumor spheroids, and mice, showcasing their potential for advanced fluorescence imaging.
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