Related Experiment Video
Updated: Sep 19, 2025
Measurement of Differentially Methylated INS DNA Species in Human Serum Samples as a Biomarker of Islet β Cell Death
Published on: December 21, 2016
The Comparison of the Gut Microbiome Composition, Serum Inflammatory Markers and Faecal Short-Chain Fatty Acids Among
Hossein Yarmohammadi1,2, Masood Soltanipur1,3, Mahdi Rezaei1,4
1Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
Background:
This study aimed to compare the gut microbiome (GM) composition, serum inflammatory markers and faecal short-chain fatty acids among individuals with type 1 and type 2 diabetes mellitus (DM) and healthy controls.
Methods:
This case-control study examined 49 subjects with type 2 DM, 21 with type 1 DM and 40 healthy controls. Blood and faecal samples were collected. Serum inflammatory markers, including CRP, IL-1β, IL-6, TNF-α and IFN-γ, were measured using enzyme-linked immunosorbent assays (ELISA). Bacterial populations were quantified using RT-qPCR and NGS. Faecal metabolites were analysed using gas chromatography.
Results:
Simpson's alpha diversity was higher among types 1 and 2 DM than in the control. The frequency of the bacterial genera Gemmiger, Dorea, Collinsella, Escherichia/Shigella, Dialister, Coprococcus, Achromobacter, Intestinimonas and Allisonella in type 2 DM was higher than in the control, and the frequency of the genera Romboutsia and Clostridium was decreased in type 2 DM. The frequency of the Prevotella, Bacteroides and Faecalibacterium genera in type 1 DM was lower than in the other groups. Acetate, propionate and butyrate levels were significantly higher in type 2 DM patients compared to the other groups. Participants with diabetes had significantly higher hs-CRP, IL1-β, TNF, IL-6 and IFG levels compared to the controls. Compared to healthy controls, both T1DM and T2DM patients showed a significant increase in the abundance of the Lactobacillus genus (p = 0.01) and a decrease in Faecalibacterium (p = 0.02). Additionally, serum levels of IL-6 and TNF-α were significantly elevated in T2DM patients (p = 0.003 and p = 0.005, respectively). Faecal levels of butyrate were significantly reduced in both diabetic groups compared to the controls (p = 0.004).
Conclusion:
By determining the GM alterations in patients with diabetes, interventional strategies could be designed to modulate the GM composition as an adjunctive therapy in diabetes.
More Related Videos
07:22Glycemic Impact on Knee Osteoarthritis Symptoms on Physical, Radiographic, and Inflammatory Markers among Individuals Aged 50 and Over with Diabetes
Published on: March 7, 2025
11:22Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Related Concept Videos
01:22Diabetes Mellitus: Type 2 and Gestational
01:22Diabetes Mellitus: Overview and Type I Subtype
Type 1 diabetes is an autoimmune disease in which the immune system mistakenly attacks and destroys the insulin-producing beta cells in the pancreas. As a result, the body is unable to produce sufficient insulin, and individuals with...
01:20Pathophysiology of Diabetes
Type 1 diabetes is characterized by autoimmune-mediated destruction of pancreatic β cells, with environmental factors potentially triggering this process in genetically susceptible individuals. Despite many not having a family history, certain genes increase susceptibility,...
01:15Diabetes: Symptoms, Diagnosis, and Complications
01:30Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
01:30Inflammatory Bowel Disease II: Crohn's Disease
Inflammatory bowel disease, commonly known as IBD, refers to a collection of disorders that lead to persistent inflammation of the gastrointestinal tract. The two types of IBD are ulcerative colitis, which impacts the colon, and Crohn's disease, which can involve any part of the gastrointestinal segment.
Crohn's disease
Crohn's disease is a chronic, systemic inflammatory bowel disease (IBD) that predominantly affects the gastrointestinal tract. It is marked by...