Related Experiment Video
Updated: Sep 19, 2025
10:59
An ex-ovo Chicken Embryo Culture System Suitable for Imaging and Microsurgery Applications
Published on: October 23, 2010
23.2K
Embryoid bodies as a model system for exploring early human embryonic development
Daria I Zhigalina1, Evgeny V Denisov2,3, Igor N Lebedev4
1Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Sciences, Ushaika Street 10, Tomsk, 634050, Russia.
Journal of Assisted Reproduction and Genetics
|June 17, 2025
Summary
Embryoid bodies (EBs) derived from pluripotent stem cells closely replicate early human embryonic development. These EBs offer a powerful model for studying embryogenesis, advancing reproductive genetics and medicine.
Area of Science:
- Developmental Biology
- Stem Cell Biology
- Reproductive Medicine
Background:
- Embryoid bodies (EBs) are three-dimensional aggregates of pluripotent stem cells.
- They mimic early stages of embryonic development post-implantation.
- Understanding EB formation and properties is crucial for developmental studies.
Purpose of the Study:
- To review current knowledge on embryoid bodies (EBs).
- To evaluate EBs as a model for human embryonic development.
- To compare embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) in EB models.
Main Methods:
- Literature review and analysis of existing concepts on embryoid bodies.
- Collection and analysis of data on EB characteristics and stem cell properties.
- Comparative analysis of ESC- and iPSC-derived EB models.
Main Results:
- EBs from pluripotent stem cells effectively model post-implantation human embryonic development.
- Comparative analysis highlights advantages and limitations of ESC vs. iPSC models.
- Limited single-cell RNA sequencing studies in human and mouse EBs are summarized.
Conclusions:
- Embryoid bodies represent a valuable in vitro model for studying early human embryogenesis.
- This model system holds significant potential for advancements in reproductive genetics and medicine.
Keywords:
Embryoid bodiesEmbryonic developmentInduced pluripotent stem cellsModel systemsSingle-cell sequencingTranscriptomeMore Related Videos
Related Concept Videos
01:56Gastrulation
58.8K
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
58.8K
01:33Cleavage and Blastulation
46.0K
After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
46.0K
01:10Zygotic Development And Stem Cell Formation
5.5K
The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
5.5K
01:29Bone Formation by Intramembranous Ossification
7.8K
Intramembranous ossification is one of the two processes involved in the development of bones within an embryo. The flat bones of the face, most of the cranial bones, and the clavicles are formed via this process. During intramembranous ossification, the bones develop directly from sheets of undifferentiated mesenchymal connective tissue.
The process begins when mesenchymal cells in the embryonic skeleton gather together and differentiate into osteogenic cells, which then develop into ...
The process begins when mesenchymal cells in the embryonic skeleton gather together and differentiate into osteogenic cells, which then develop into ...
7.8K
01:15Development of the Sexual Organs in the Embryo and Fetus
1.5K
Development of the reproductive organs in an embryo starts from a bipotential state. This means the early embryo can develop either male or female reproductive organs. The formation of these organs begins with the growth of gonadal ridges that arise from the intermediate mesoderm during the fifth week of development.
Near the gonadal ridges, two duct systems are present: the mesonephric ducts (Wolffian ducts) and paramesonephric ducts (Müllerian ducts). These ducts form the basis for the...
Near the gonadal ridges, two duct systems are present: the mesonephric ducts (Wolffian ducts) and paramesonephric ducts (Müllerian ducts). These ducts form the basis for the...
1.5K