Related Experiment Video

Updated: Sep 19, 2025

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders
10:21

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Published on: October 7, 2018

9.7K

Parental origin of transgene determines recombination efficiency in GFAP-creERT2 mice

Karmen Mah1, Adam Roszczyk1, Ashley E Frakes1,2

  • 1Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health, Bethesda, MD 20892, USA.

G3 (Bethesda, Md.)
|June 17, 2025
PubMed

Keywords:
CreCreERT2astrocytesinheritancematernalmiceneurosciencerecombination

More Related Videos

Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain09:44

Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain

Published on: May 20, 2022

3.2K
Clonal Genetic Tracing using the Confetti Mouse to Study Mineralized Tissues07:41

Clonal Genetic Tracing using the Confetti Mouse to Study Mineralized Tissues

Published on: October 23, 2019

14.7K

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.2K
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.9K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
14.2K