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Related Experiment Video

Updated: Sep 19, 2025

Using R, Seurat, and CellChat to Analyze a Single-Cell Transcriptomics Dataset of Mouse Skin Wound Healing
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Single-Cell Analysis Reveals Fibroblast-Derived Migrasomes as CXCL12 Carriers Promoting Skin Wound Repair.

Haoyu Zhou1, Zhen Zhang1,2, Zhan Liu1

  • 1State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, China.

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|June 17, 2025
PubMed
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Migrasomes, released by fibroblasts, significantly accelerate skin wound healing by delivering essential proteins. These newly discovered organelles show promise as therapeutic vesicles for tissue repair.

Keywords:
extracellular vesiclesmigrasomesingle‐cell RNA sequenceskin wound healing

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Area of Science:

  • Cell Biology
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Migrasomes are novel organelles implicated in morphogenesis and angiogenesis.
  • Their role in tissue repair, particularly skin wound healing, remains largely unexplored.

Purpose of the Study:

  • To investigate the function of migrasomes in skin and oral mucosa tissue repair.
  • To determine the therapeutic potential of fibroblast-derived migrasomes in accelerating wound healing.

Main Methods:

  • Super-resolution confocal microscopy and focused ion beam scanning electron microscopy to visualize migrasomes.
  • Live-cell imaging and multiplex immunofluorescence staining to track migrasome origin and uptake.
  • In vivo wound healing assays in rats and in vitro cell culture experiments.

Main Results:

  • Migrasomes were observed connected to fibroblast-derived retraction fibers and released contents in skin and oral mucosa.
  • Fibroblast-derived migrasomes were taken up by fibroblasts and keratinocytes (HaCaT cells).
  • Injection of purified migrasomes accelerated wound healing in rats, upregulating key healing factors like VEGFA, IL-6, and COL1A1.

Conclusions:

  • Fibroblast-derived migrasomes play a direct role in promoting skin wound healing.
  • Migrasomes act as therapeutic vesicles, delivering factors like CXCL12 to enhance tissue repair.
  • These findings highlight migrasomes as a potential novel therapeutic strategy for skin regeneration.